申请/专利权人：加利福尼亚大学董事会

申请日：2017-11-16

公开（公告）日：2024-06-07

公开（公告）号：CN110139927B

主分类号：C12N5/0789

分类号：C12N5/0789;C12N5/10;C12N9/22;C12N15/63;C12N15/79;C12N15/85;C07K14/195

优先权：["20161116 US 62/422,850"]

专利状态码：有效-授权

法律状态：2024.06.07#授权;2019.12.06#实质审查的生效;2019.08.16#公开

摘要：提供了Cas9抑制性多肽组合物和方法。

主权项：1.一种抑制细胞中Cas9多肽的方法，其中所述方法用于非治疗目的，所述方法包括，将Cas9抑制性多肽引入细胞，其中：所述Cas9抑制性多肽对于所述细胞而言是异源性的，并且所述Cas9抑制性多肽是SEQIDNO:147的氨基酸序列；由此抑制细胞中的Cas9多肽。

全文数据：CRISPR-CAS9抑制剂相关专利申请的交叉参考本申请要求2016年11月16日提交的美国临时专利申请号62422,850的优先权，其通过引用纳入本文用于所有目的。背景技术防止病毒攻击的能力是细胞生命的标志。细菌采用多种机制抵抗细菌病毒噬菌体的感染，包括限制酶和CRISPR-Cas系统Labrie,S.J.,Samson,J.E.和Moineau,S.2010.NatRevMicro,8,317–327。由于其规律成簇的间隔短回文重复之间有小的间隔子序列，CRISPR阵列具有先前与可动遗传因子相遇的序列特异性残余Mojica,F.J.M等.2005.J.Mol.Evol.,60,174–182。采用这些间隔子来产生向导RNA，它们促进经编程靶标的结合与切割Brouns,S.J.J等.2008.Science,321,960–964；Garneau,J.E.等.2010.Nature,468,67–71。免疫功能所需的CRISPR相关的cas基因常存在于CRISPR阵列附近Marraffini,L.A.2015.原核生物中的CRISPR-Cas免疫CRISPR-Casimmunityinprokaryotes.Nature,526,55–61；Wright,A.V.,J.K.和Doudna,J.A.2016.Cell,164,29–44。Cas蛋白不仅进行外来基因组的破坏Garneau,J.E.等.2010.Nature,468,67–71，还促进成熟CRISPRRNAcrRNAs的生成Deltcheva；Haurwitz,R.E等.2010.Science,329,1355–1358以及将外来序列收进CRISPR阵列J.K.等.2014.Nat.Struct.Mol.Biol,21,528-534；Yosef,I.,Goren,M.G.和Qimron,U.2012.NucleicAcidsResearch,40,5569–5576。CRISPR-Cas适应性免疫系统在细菌世界中是常见且多样的。细菌基因组中已鉴定出六种不同类型I-VIAbudayyeh,O.O等.2016.Scienceaaf5573；Makarova,K.S.等.2015.NatRevMicro,13,722–736.NatRevMicro,13,722–736，其有能力按RNA向导的指定切割靶DNA或RNA序列。CRISPR-Cas系统的简易可编程性已得到广泛开发，为许多新型遗传技术打开了大门Barrangou,R.和Doudna,J.A.2016，NatureBiotechnology,34,933–941。这些技术中的大多数使用来自酿脓链球菌Streptococcuspyogenes，Spy的Cas9，以及经工程改造的单向导RNA作为此类应用的基础，包括动物细胞中的基因编辑Cong,L.等.2013.Science339,819–823；Jinek,M.等2012.Science,337,816–821；Mali,P.等2013.Science,339,823–826；Qi,L.S.等2013.Cell,152,1173–1183。此外，已经研究了II-A亚型内的Cas9直系同源物用于基因编辑应用Ran,F.A.等2015.Nature520,186–191，并且新2类CRISPR单蛋白效应物如Cpf1V型Zetsche,B.等2015.Cell,163,759–771和C2c2VI型Abudayyeh,O.O等2016.Scienceaaf5573；East-Seletsky,A.等2016.Nature538,270-273正在表征中。1类CRISPR-Cas系统I、III和IV型是RNA引导的多蛋白复合物，因此它们因其复杂性而被大多数基因组应用忽视。然而，这些系统在近一半的细菌和约85％的古细菌中是最常见的Makarova,K.S.等2015.NatRevMicro,13,722–736.NatRevMicro,13,722–736。为了应对细菌对噬菌体感染的战争，噬菌体反过来经常编码细菌免疫系统的抑制剂，这增强了它们裂解宿主细菌或整合到其基因组中的能力Samson,J.E.等2013.NatRevMicro,11,675–687。噬菌体编码的“抗CRISPR”蛋白的第一个例子来自铜绿假单胞菌Pseudomonasaeruginosa的1类I-E和I-F型系统Bondy-Denomy等2013.Nature,493,429–432；Pawluk,A.等2014.mBio5,e00896。值得注意的是，到目前为止已经发现了十种I-F型抗CRISPR和四种I-E型抗CRISPR基因Pawluk,A.等2016.NatureMicrobiology,1,1–6，所有这些都编码不同的小蛋白质50-150个氨基酸，先前功能未知。我们对4种I-F抗CRISPR蛋白的生化研究表明，它们与多蛋白CRISPR-Cas复合物中的不同Cas蛋白直接相互作用，以防止靶DNA的识别或切割Bondy-Denomy,J等2015.Nature,526,136–139。各蛋白质具有不同序列、结构和作用模式Maxwell,K.L.等2016.NatureCommunications,7,13134；Wang,X.2016.Nat.Struct.Mol.Biol23,868-870。这些发现支持CRISPR-Cas抑制剂的独立进化，并表明还有更多尚未发现。鉴于此，近期的一篇论文利用了具有预测的螺旋-转角-螺旋HTH基序的特征性抗CRISPR相关aca基因的保守性来鉴定铜绿假单胞菌P.aeruginosa外的抗CRISPR基因。这导致作者在变形菌门中发现抗CRISPR，其广泛地跨越I-F型CRISPR-Cas系统发育Pawluk,A.等2016.NatureMicrobiology,1,1–6。这表明所有CRISPR系统都可能存在抗CRISPR，并且需要能够发现它们的方法。铜绿假单胞菌中的I型抗CRISPR从整合的噬菌体基因组原噬菌体表达，导致宿主CRISPR-Cas系统的组成型失活。Bondy-Denomy等2013.Nature,493,429–432。这通常会导致原噬菌体具有与同一细胞中共同出现的CRISPR间隔子完全一致的DNA靶标的情况，称为“自靶向”图1A。这种情况使得CRISPR-Cas灭活成为生存的必要条件，因为在没有原噬菌体抗CRISPR基因的情况下，宿主基因组在靶向原噬菌体的过程中被切割Bondy-Denomy等2013.Nature,493,429–432；Edgar,R.和Qimron,U.2010.J.Bacteriol192,6291–6294。抗CRISPR的表达会中和该风险，然而，这将允许溶素原存活。我们推测，具有明显自靶向的CRISPR系统的基因组将是鉴定新CRISPR-Cas抑制剂的候选者。我们在此描述了使用生物信息学方法在单核细胞增生李斯特菌Listeriamonocytogenes中鉴定以前未知的噬菌体编码的CRISPR-Cas9抑制剂，以识别自靶向事件。我们显示这些抑制剂中的两种也可以阻断酿脓链球菌Cas9在细菌细胞和人细胞中的活性。定义术语“核酸”或“多核苷酸”是指单链或双链形式的脱氧核糖核酸DNA或核糖核酸RNA及其聚合物。除非特别限定，否则该术语涵盖含有天然核苷酸的已知类似物的核酸，其具有与参比核酸相似的结合特性，并以与天然存在的核苷酸相似的方式代谢。除非另有说明，否则具体核酸序列还隐含地包括其保守修饰的变体例如，简并密码子取代，等位基因，直系同源物，SNP和互补序列以及明确指出的序列。具体地，简并密码子取代可以通过产生其中一个或多个选定或所有密码子的第三位被混合碱基和或脱氧肌苷残基取代的序列来实现Batzer等,NucleicAcidRes.19:50811991；Ohtsuka等,J.Biol.Chem.260:2605-26081985；和Rossolini等,Mol.Cell.Probes8:91-981994。术语“基因”是指参与产生多肽链的DNA区段。它可以包括编码区之前和之后的区域前导区和尾区以及各个编码区段外显子之间的间插序列内含子。“启动子”定义为指导核酸转录的核酸控制序列的阵列。如本文所用，启动子包括转录起始位点附近的必需核酸序列，例如，在聚合酶II型启动子的情况中，TATA元件。启动子还任选地包括远端增强子或阻遏子元件，其可位于距转录起始位点数千碱基对的位置。启动子可以是异源性启动子。“表达盒”是重组或合成产生的核酸构建体，具有一系列允许特定多核苷酸序列在宿主细胞中转录的特定核酸元件。表达盒可以是质粒，病毒基因组或核酸片段的一部分。通常，表达盒包括待转录的多核苷酸，其操作性地连接至启动子。启动子可以是异源性启动子。在与多核苷酸操作性地连接的启动子的情况中，“异源性启动子”是指不与自然产物中例如，在野生型生物中存在的相同多核苷酸如此操作性地连接的启动子。“多肽”，“肽”和“蛋白质”在本文中可互换使用，指氨基酸残基的聚合物。三个术语全部适用于其中一个或多个氨基酸残基是相应天然产生氨基酸的人造化学模拟物的氨基酸聚合物，以及天然产生的氨基酸聚合物和非天然产生的氨基酸聚合物。如本文所用，该术语涵盖任意长度的氨基酸链，包括全长蛋白质，其中所述氨基酸残基通过共价肽键连接。“保守修饰的变体”适用于氨基酸和核酸序列。关于特定的核酸序列，“保守修饰的变体”是指那些编码相同或基本相同的氨基酸序列的核酸，或者当核酸不编码氨基酸序列时，指的是基本相同的序列。由于遗传密码的简并性，大量功能相同的核酸编码任何给定的蛋白质。例如，密码子GCA、GCC、GCG和GCU都编码氨基酸丙氨酸。因此，在密码子指定丙氨酸的每个位置，该密码子可以改变为所述的任何相应密码子而不改变编码的多肽。这种核酸变异是“沉默变异”，其是保守修饰变异的一种。本文中编码多肽的每个核酸序列也描述了核酸的每种可能的沉默变异。本领域技术人员应认识到，核酸中的每个密码子除了AUG，其通常是甲硫氨酸的唯一密码子，和TGG，其通常是色氨酸的唯一密码子均可以被修饰以产生功能相同的分子。因此，编码多肽的核酸的每个沉默变异隐含在每个所描述的序列中。至于氨基酸序列，本领域技术人员应认识到，核酸、肽、多肽或蛋白质序列的单个取代、缺失或添加其改变、添加或删除编码序列中的单个氨基酸或少量氨基酸是“保守修饰的变体”，其中所述改变导致被化学上相似的氨基酸取代的氨基酸取代。提供功能上相似氨基酸的保守取代表是本领域熟知的。此类保守修饰的变体是本发明的多态性变体、种间同源物和等位基因的补充且并不排除它们。在一些情况下，保守修饰的Cas9或sgRNA变体可具有如本文所述的增加的稳定性、组装或活性。以下八组每组含有相互保守取代的氨基酸：1丙氨酸A，甘氨酸G；2天冬氨酸D，谷氨酸E；3天冬酰胺N，谷胺酰胺Q；4精氨酸R，赖氨酸K；5异亮氨酸I，亮氨酸L，甲硫氨酸M，缬氨酸V；6苯丙氨酸F，酪氨酸Y，色氨酸W；7丝氨酸S，苏氨酸T；和8半胱氨酸C，甲硫氨酸M参见，例如，Creighton，《蛋白质》Proteins,弗里曼公司W.H.FreemanandCo.，纽约1984。在本文中，氨基酸可用IUPAC-IUB生物化学命名委员会推荐的通用三字母符号或单字母符号表示。同样，核苷酸可由其公认的单字母代码表示。在本申请中，氨基酸残基根据它们在未修饰的野生型多肽序列中从最左边的残基编号为1的相对位置编号。如本文所用，在描述两个或更多个多核苷酸或氨基酸序列的上下文中，术语“相同”或“相同性”百分比是指两个或更多个相同的序列或指定的子序列。当比较和比对以在比较窗口上进行最大对应，或使用序列比较算法或通过手动比对和目视检查测量的指定区域其中未指定特定区域时，两个“基本相同”的序列具有至少60％的相同性，优选65％，70％，75％，80％，85％，90％，91％，92％，93％，94％，95％，96％，97％，98％，99％或100％相同性。关于多核苷酸序列，该定义还指测试序列的互补序列。关于氨基酸序列，在一些情况下，相同性存在于长度为至少约50个氨基酸或核苷酸的区域上，或更优选地长度为75-100个氨基酸或核苷酸的区域上。为了序列比较，一般将一个序列用作与测试序列比较的参比序列。当使用序列比较算法时，将测试和参比序列输入计算机，如果需要，指定子序列坐标，并指定序列算法程序参数。可使用默认的程序参数，或者可指定另外的参数。然后，序列比较算法基于程序参数计算测试序列相对于参比序列的序列相同性百分比。对于核酸和蛋白质的序列比较，使用BLAST2.0算法和下面讨论的默认参数。如本文所用，“比较窗口”包括对于选自20至600，通常约50至约200，更通常约100至约150个的多个邻接位置中的任何一个的区段的参考，其中，在两个序列最佳比对后，可以将序列与连续位置的相同数目的参比序列进行比较。用于确定序列相同性百分比和序列相似性的算法是BLAST2.0算法，其描述于Altschul等,1990J.Mol.Biol.215:403-410。用于进行BLAST分析的软件可在国家生物技术信息中心网站ncbi.nlm.nih.gov上公开获得。此算法包括：首先通过鉴定查询序列中长度为W的短字来鉴定高评分序列对HSP，与数据库序列中长度相同的字比对时它们能匹配或满足一些正值的阈值评分T。T称为相邻字评分阈值Altschul等，同上。这些初始相邻字命中wordhit用作启动搜索的种子，以便找到含有它们的较长HSP。然后，沿各序列在两个方向上延伸该字命中，直到提高累积的比对评分。就核苷酸序列而言，采用参数M一对匹配残基的奖励评分；总是0和N错配残基的罚分；总是40％蛋白质序列相同性的核心噬菌体基因以外，还鉴定了10个非重叠的独特基因簇基于预测的操纵子结构选择簇边界，每簇1-12个基因。克隆每个簇并整合到原噬菌体治愈型10403s的基因组中并测定CRISPR-Cas9功能。在10个片段中，7个成功地引入单核细胞增生李斯特菌，而3个片段不能插入到单核细胞增生李斯特菌基因组中，并且可能在分离时具有毒性。质粒转化测定显示φJ0161a片段1是唯一能够抑制CRISPR-Cas9的片段，表明该片段编码至少一种CRISPR-Cas9抑制剂图2D，图8。表达来自该四基因片段的个体基因导致两个抗CRISPR基因LMOG_03146和LMOG_03147的确定鉴定本文分别称为acrIIA1和acrIIA2；图2B和2D。10403s::ΦJ0161a溶素原中acrIIA1和acrIIA2的缺失恢复了CRISPR-Cas9功能，证实这些是ΦJ0161a中仅有的抗CRISPR基因图2B。抗CRISPR基因座在单核细胞增生李斯特菌中广泛存在为了鉴定其它II-A型抗CRISPR，检测了相关的单核细胞增生李斯特菌原噬菌体中与acrIIA1和acrIIA2类似的基因组位置。在留下的整合位点和参与细胞裂解的基因之间鉴定出在具有高度保守的基因顺序的小操纵子2-5个基因内含有acrIIA1的重复的抗CRISPRacr基因座图3A。我们鉴定了acr基因座内保守的5个新蛋白质家族。为了测试这些，我们将代表物质克隆并整合到10403s基因组中并测定pT和pNT的转化效率。鉴定了两种能够使CRISPR失活的新基因acrIIA3和acrIIA4，而其余三种orfC，D，E则不是图3A，图9。为了确定单核细胞增生李斯特菌中CRISPR-Cas9的失活是否普遍存在，我们接下来分析了每种抗CRISPR的保守模式。虽然每个acrIIA基因足以使分离物中的CRISPR-Cas9失活，但我们观察到在大多数acr基因座中acrIIA1的常见存在。在含有螺旋-转角-螺旋HTH基序的acrIIA1的上游发现几乎所有实例91％的acrIIA2-4，表明该基因可能是acr基因座的标志物图3B。我们在119个acr基因座中观察到的最常见的情况是acrIIA1-2或acrIIA1-2-3，代表66％的acr基因座。总共在25％的单核细胞增生李斯特菌基因组中鉴定出acrIIA基因，其中约50％的含有单核细胞增生李斯特菌cas9的菌株在相同的基因组中具有至少一个抗CRISPR图3C。还鉴定了许多具有多种acrIIA编码原噬菌体的单核细胞增生李斯特菌基因组的实例补充表1。此外，在最初鉴定的所有八个自靶向实例的基因组中发现至少一个acrIIA基因图1B，补充表1，解释了这些情况如何稳定。总之，这些数据表明在单核细胞增生李斯特菌中普遍存在原噬菌体介导的CRISPR-Cas9失活。表S1.acrIIA基因保守性，图1B4B和4C相关先前含有HTH的抗CRISPR相关aca基因被用作鉴定新型I型抗CRISPR基因的标志物Pawluk,A.等.2016.NatureMicrobiology,1,1–6，尽管aca基因本身没有抗CRISPR活性。我们假设acrIIA1可以发挥这种标志物的作用。对acrIIA1进行了全面的进化分析，揭示了在厚壁菌中在可动元件和核心基因组中广泛检测到的同源物图4A。在李斯特菌基因组中鉴定了一系列远缘相关的acrIIA1同源物，例如orfD，其是已经独立鉴定为acr基因座成员的含HTH的基因图3A。虽然该基因在功能测定中缺乏抗CRISPR活性，但其与质粒pLMIV中的acrIIA4的共同出现表明广泛的acrIIA1orfD超家族可用作鉴定新acr基因的标志物图3A。未来的工作对于确定这些系统中含有HTH的基因是否可作为新型抗CRISPR发现的有效标志物是必要的。为了确定acrIIA2-4的同源性，进行了类似的进化分析。与在厚壁菌核心基因组中广泛存在的acrIIA1不同，这些其它三种acr基因大多局限于李斯特菌中的原噬菌体。鉴定了三个不同的acrIIA2序列家族，仅在李斯特菌长尾噬菌体siphophages一个长尾，非收缩噬菌体家族中发现图4B，而在李斯特菌和链球菌的长尾噬菌体基因组中观察到两个acrIIA3家族图4C。最后，在两个不同的序列家族中观察到acrIIA4，一个在李斯特菌长尾噬菌体和质粒中，另一个在一组专性毒性噬菌体长收缩尾噬菌体中观察到图4D。虽然acrIIA2和acrIIA3几乎总是与acrIIA1一起发现，但acrIIA4经常在缺乏李斯特菌的可动元件和噬菌体中的acrIIA1同源物时发生。例如，毒性噬菌体中的acrIIA4家族是不同的，因为它们在预测的蛋白质中具有约70个氨基酸的C末端延伸，并且不与含有HTH的基因acrIIA1或orfD一起出现，表明这些acrIIA4家族之间存在潜在的机制和进化差异。总之，这些分析揭示了用于检测同源性acr基因的特异性决定因子的充足序列空间，并且提示了在单核细胞增生李斯特菌中cas9和可动元件之间的活跃的竞争。AcrIIA2、AcrIIA3和AcrIIA4抑制酿脓链球菌Cas9为了确定LmoCas9AcrIIA蛋白的多功能性，我们研究这些抑制剂对来自酿脓链球菌的相关Cas9蛋白是否起作用Spy，与LmoCas953％相同。该直系同源物已被广泛用于生物技术应用，作为RNA引导的核酸酶Barrangou,R.和Doudna,J.A.2016,NatureBiotechnology,34,933–941，以及用于通过用于可编程基因抑制的催化失活的突变体dCas9进行的可编程基因抑制Gilbert,L.A.等.2013.Cell154,442-451；Qi,L.S.等.2013.Cell,152,1173–1183。使用携带SpydCas9的大肠杆菌菌株，我们测试了AcrIIA蛋白是否阻断dCas9干扰染色体RFP报告基因的转录图5A。在缺乏抑制剂的遗传背景中，相对于没有向导RNA的菌株，sgRNA和dCas9的存在将RFP荧光降低至2.6％。acrIIA2部分阻断dCas9功能，荧光上升至25％，而acrIIA4几乎完全阻断dCas9，具有无导向对照的荧光的85％图5B。我们无法从acrIIA3获得有意义的数据，因为该蛋白质对大肠杆菌有毒。这在流式细胞术期间使记录的细胞计数降低参见图10a并导致荧光测量的较大变异性。还测试了来自酿脓链球菌的AcrIIA3的同源物登录号：AND04610.1，其与Lmo_acrIIA3具有45％的序列相同性，但导致大肠杆菌的生长受损图10b。acrIIA3毒性的机制仍有待确定。我们得出结论，acrIIA2和acrIIA4在不同程度上抑制大肠杆菌中的SpydCas9。鉴于SpyCas9在真核细胞中的普遍应用，我们接下来测试了AcrIIA蛋白在人体细胞中阻断基因编辑的能力。在存在或不存在表达人密码子优化的AcrIIA蛋白的载体的情况下，用表达SpyCas9和靶向eGFP的sgRNA的质粒瞬时转染具有诱导型eGFP报告基因的HEK293T细胞。在允许基因编辑进行36小时后，诱导eGFP12小时，然后通过流式细胞术测量细胞荧光图5C。在Cas9和eGFPsgRNA存在下，基因编辑导致GFP阳性细胞数量减少25％，而与acrIIA2或acrIIA4的共表达阻止了基于Cas9的基因编辑图5D。我们在该测定中另外测试了acrIIA3Spy_acrIIA3的酿脓链球菌同源物，其在人细胞中无毒，但对Cas9功能没有影响。acrIIA1在人细胞中是无功能的，orfA也是，其为一种在单核细胞增生李斯特菌中没有抗CRISPR活性的阴性对照。与大肠杆菌中的dCas9实验一起，这些数据证明了AcrIIA2和AcrIIA4蛋白在异源宿主中抑制直系同源Cas9的功能的效用。因此，这些试剂代表CRISPR-Cas9基因组工程改造工具包中的新工具。讨论噬菌体编码的细菌免疫系统抑制剂由于这两个实体之间的进化竞争中的强选择压力而出现Samson,J.E.等.2013.NatRevMicro,11,675–687。I型CRISPR-Cas系统中噬菌体编码的抗CRISPR的首次鉴定暗示存在更多的CRISPR抑制剂，但缺乏其发现的方法。本文中，我们提出了一种生物信息学策略，该策略使用“自靶向”作为CRISPR-Cas抑制剂基因的基因组标志物图1A。该方法导致鉴定出四种不同的II-A型CRISPR-Cas9抑制剂图3A，它们共同存在于所有编码Cas9的单核细胞增生李斯特菌基因组的一半中，包括具有自靶向的所有基因组图3C。我们预计这种方法将有助于鉴定其它CRISPR-Cas系统中的acr基因，尽管已经描述了III型系统的自靶向耐受的独特机制Goldberg,G.W.等.2014.Nature514,633–637；Samai,P.等.2015.Cell,161,1164–1174。为了便于鉴定AcrIIA蛋白，我们首先在单核细胞增生李斯特菌中证明了功能性CRISPR-Cas9系统图2B。先前对该生物体中CRISPR-Cas的研究集中于IB型系统和缺乏cas基因的相关IB衍生的CRISPR孤儿阵列Mandin,P.等.2007.NucleicAcidsResearch,35,962–974；Sesto,N.等.2014.PLoSGenet,10,e1004065。虽然之前没有为这两种系统建立规范的CRISPR-Cas功能，但是显示孤儿阵列由宿主核糖核酸酶处理以产生非编码RNAMandin,P.等2007.NucleicAcidsResearch,35,962–974；Sesto,N.等2014.PLoSGenet,10,e1004065。为了观察II-ACRISPR-Cas系统的功能，我们使用标准转化效率测定法，显示菌株10403s中的Cas9功能足以限制质粒以序列特异性方式转化图2A-C。鉴于在用pT转化10403s期间观察到的小集落表型，我们怀疑内源性cas9表达水平不足以完全清除质粒，导致质粒保持在微小的集落中。确认这一点，cas9的表达增加导致该测定中转化体完全消除图2C。鉴于φJ0161a可以抑制这种过表达的CRISPR-Cas9系统图2C，我们得出结论，鉴定的抑制剂能够阻断内源性和过表达的Cas9功能。为了鉴定候选抗CRISPR基因，比较了来自CRISPR活性菌株10403s的相关原噬菌体与抑制来自菌株J0161的CRISPR的原噬菌体，并且采用了消除克隆方法的操作图2D。确定了存在于φJ0161中的acrIIA1和acrIIA2基因的两个分离的acr基因的鉴定。在寻找更多抗CRISPR中，我们发现相关噬菌体中保守的基因组定位是鉴定不同II-A型Cas9抑制蛋白的良好代表，尽管蛋白质本身之间缺乏序列保守性图3A。先前已在IF和IE型抗CRISPR的研究中观察到这种情况Bondy-Denomy等2013.Nature,493,429–432；Pawluk,A.等2014.mBio5,e00896。在单核细胞增生李斯特氏菌中，原噬菌体中Cas9抑制剂的高流行性表明CRISPR-Cas9功能的广泛失活图3C。目前，我们不了解是否存在机制关联来解释acrIIA1与其它抗CRISPR的常见共存图3A和3B。尽管该基因足以在质粒攻击测定中使CRISPR-Cas9功能失活，但我们推测其可在感染或溶原过程中充当其它acrIIA基因的辅因子或调节因子，因此解释了观察到的基因组关联。未来的工作将有必要了解AcrIIA1实际上是否是这方面的双功能蛋白，更广泛地说，超家族是否是acr基因的标志物。进化分析证明了李斯特菌可动元件中可动元件中acrIIA2-4的常见发生图4。这可能通过阻断基于Cas9的靶向和适应来促进该生物体中的水平基因转移Heler,R.等.2015Nature519,199–202。除了首次鉴定出这些acrIIA基因的原噬菌体家族外，还在较远的长尾噬菌体、肌尾噬菌体myophage和质粒中发现了同源物。最值得注意的是，由强毒性肌尾噬菌体编码的acrIIA4同源物在其附近没有acrIIA1超家族同源物。此外，在李斯特菌属之外的属中存在acrIIA1和acrIIA3同源物表明CRISPR-Cas9失活可能在厚壁菌门中是常见的。为了使CRISPR-Cas9功能失活，理论上许多机制都是可能的。通过证明acrIIA2和acrIIA4在具有工程改造的元件即cas9启动子，sgRNA设计和启动子的异源宿主中的功效，我们得出结论，转录抑制是不可能的。I型抗CRISPR的功能是通过直接结合干扰所需的Cas蛋白并阻止DNA结合或DNA切割Bondy-Denomy,J等.2015.Nature,526,136–139。通过扩展，我们期望acrIIA2和acrIIA4具有类似的机制，因为它们具有在人细胞中发挥作用的能力。相对于基于dCas9的测定，acrIIA2在人细胞中基于裂解的Cas9测定中增强的功效表明它可以在一定程度上抑制结合和切割，其中切割抑制表现为Cas9功能的完全失活。在AcrIIA4的情况下，DNA结合明显被抑制，尽管这是否通过与Cas9的直接相互作用仍有待观察。II-A型抑制剂的鉴定和未来机制解析将为在自然和工程化环境中研究经典CRISPR-Cas9功能提供有价值的新试剂。AcrIIA蛋白在大肠杆菌和人细胞中阻断SpyCas9的能力表明这些蛋白质可以为Cas9提供翻译后“关闭”。这可以为这个功能强大的系统增加一层调节，可以应用于真核系统来控制基因组工程改造。CRISPR-Cas9工具箱的这一新增功能可以实现新的应用，例如特异性逆转dCas9与基因组基因座结合的影响，或限制Cas9在细胞核中活化的时间量，以减少脱靶基因编辑。重要的是扩大对抑制蛋白的搜索，以继续利用从噬菌体-细菌竞赛中提供给我们的丰富工具。表S2.菌株与质粒,实验模型与主题详细信息微生物单核细胞增生李斯特菌菌株在脑心浸液BHI培养基上培养。大肠杆菌菌株在LB培养基上培养。细胞系人胚胎肾293加T细胞抗原HEK293T，CRL-3216，ATCC细胞在补充有10％胎牛血清FBS，AtlantaBiologicals和50μgmL青霉素链霉素PS，UCSFCCF的杜氏改良伊氏培养基DMEM中培养。实施例2我们试图鉴定acrIIA1-4图13的同源物，其具有针对Cas9的酿脓链球菌直系同源物的抗CRISPR功能，其广泛用于基因编辑。图14：AcrIIA1在异源系统即天然生物体外中具有抗Cas9活性。以前，该蛋白质在大肠杆菌或人类细胞中不具有抗Cas9功能。这种差异的原因尚不清楚，但与阳性对照AcrIIA4相比，AcrIIA1在这种异源系统铜绿假单胞菌中的功能非常好，同时靶向Cas9。图15：通过序列比对鉴定AcrIIA2同源物，以广泛地采样天然序列空间。除了原始蛋白质之外，还测试了三种同源物，并且如图16中的噬菌体噬斑测定所示：与原始蛋白质AcrIIA2a相比，AcrIIA2b.1和AcrIIA2b.3具有强的抗SpyCas9活性。图17：进行类似的同源性检索以鉴定AcrIIA3同源物，AcrIIA3b.2,3b.3,3b.4。如图18所示，采用其中细菌点在平板上的测定，这三种新的抗CRISPR蛋白质无毒并且在细菌中稳健有效。如本文所鉴定的，AcrIIA4是一种强大且广谱的Cas9抑制剂，其与Cas9紧密结合参见，Dong等，Nature，和Shin等，ScienceAdvances。鉴定出一种同源物AcrIIA4b，其具有中度的抗Cas9活性。图19汇总了实施例2中所述的结果。参考文献Abudayyeh,O.O等.2016.Scienceaaf5573Barrangou,R.和Doudna,J.A.2016,NatureBiotechnology,34,933–941Biswas,A.等.2013.RNABiol,10,817–827Biswas,A等.2016.BMCGenomics,17,356Bondy-Denomy等.2013.Nature,493,429–432Bondy-Denomy,J等.2015.Nature,526,136–139Brouns,S.J.J等.2008.Science,321,960–964Camilli,A.,Tilney,L.G.和Portnoy,D.A.1993.MolecularMicrobiology,8,143–157Choi,K.-H.等.2005.Nat.Methods,2,443–448Cong,L.等.2013.Science339,819–823Deltcheva,E.等.2011.Nature471,602–607Di,H.等.2014BiochemBiophysResCommun,454,399–403East-Seletsky,A.等.2016.Nature538,270-273Edgar,R.和Qimron,U.2010.192,6291–6294Edgar,R.C.2004.NucleicAcidsResearch,32,1792–1797Garneau,J.E.等.2010.Nature,468,67–71Gilbert,L.A.等.2013.Cell154,442-451Goldberg,G.W.等.2014.Nature514,633–637Grissa,I.,Vergnaud,G.和Pourcel,C.2007.NucleicAcidsResearch,35,W52–W57Guzman,L.M等.1995.J.Bacteriol.,177,4121–4130Haldimann,A.和Wanner,B.L.2001.J.Bacteriol.,183,6384–6393Haurwitz,R.E等.2010.Science,329,1355–1358Heler,R.等.2015.Nature519,199–202Jinek,M.等.2012.Science,337,816–821Labrie,S.J.,Samson,J.E.和Moineau,S.2010.NatRevMicro,8,317–327Lauer,P.等.2002.J.Bacteriol.,184,4177–4186Loessner,M.J.和Busse,M.1990.AppliedandEnvironmentalMicrobiology,56,1912–1918Makarova,K.S.等.2015.NatRevMicro,13,722–736Mali,P.等.2013.Science,339,823–826Mandin,P.等.2007.NucleicAcidsResearch,35,962–974Marraffini,L.A.2015.原核生物中的CRISPR-Cas免疫CRISPR-Casimmunityinprokaryotes.Nature,526,55–61Maxwell,K.L.等.2016.NatureCommunications,7,13134Mojica,F.J.M等.2005.J.Mol.Evol.,60,174–182J.K.等.2014.Nat.Struct.Mol.Biol21,528-534Park,S.F.和Stewart,G.S.1990.Gene,94,129–132Pawluk,A.等.2014.mBio5,e00896Pawluk,A.等.2016.NatureMicrobiology,1,1–6Qi,L.S.等.2013.Cell,152,1173–1183Ran,F.A.等.2015.Cas9.520,186–191Samai,P.等.2015.Cell,161,1164–1174Samson,J.E.等.2013.NatRevMicro,11,675–687Sesto,N.等.2014.PLoSGenet,10,e1004065J.,Biegert,A.和Lupas,A.N.2005.NucleicAcidsResearch,33,W244–W248Tamura,K.等.2013.Mol.Biol.Evol.,30,2725–2729Typas,A.等.2008.Nat.Methods,5,781–787Volz,S.E.等.2015.Cell,163,759–771Wang,X.2016.Nat.Struct.Mol.Biol23,868-870Wright,A.V.,J.K.和Doudna,J.A.2016.Cell,164,29–44Yosef,I.,Goren,M.G.和Qimron,U.2012.NucleicAcidsResearch,40,5569–5576Zemansky,J.等.2009.J.Bacteriol.,191,3950–3964Zetsche,B.等.2015.Cell,163,759–771应理解，本文所述的实施例和实施方式仅用于说明目的，本领域技术人员应了解据此作出的各种修饰或改变，且它们包括在本申请的主旨和权益以及所附权利要求书的范围内。本文引用的所有发表物、专利和专利申请通过引用全文纳入本文以用于所有目的。序列AcrIIA1蛋白序列SEQIDNO:1WP_003722518.1LMOG_03146MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNIKKDVCKALENAITVLKEKKNELLSEQIDNO:2WP_060571535.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESTNIEVLPFTFNRFENEEHVNIKKDVCKALENAITVLKEKKNELLSEQIDNO:3WP_070783094.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESNIEVLPFTFNRFENEEHVNIGKDVCKALENAITVLKEKKNELLSEQIDNO:4WP_031669445.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNIEKDVCKALENAITVLKEKKNELLSEQIDNO:5WP_070286809.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESNIEVLPFTFNRFENEKHVNIKKDVCKALENAITVLKEKKNELLSEQIDNO:6WP_070213372.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLNKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNIEKDVCKALENAITVLKEKKNELLSEQIDNO:7WP_003731275.1MAIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEKHVNIKKDVCKALENAITVLKEKKNELLSEQIDNO:8WP_010989942.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNIEKDVCKALENAITVLKEKKNELLSEQIDNO:9WP_070286796.1MKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEKHVNIKKDVCKALENAITVLKEKKNELLSEQIDNO:10WP_038409766.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLNKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELLSEQIDNO:11WP_060595919.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:12WP_047934203.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKSLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLGKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:13YP_009044824.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLTFTFNRFENEEHADIEKDVKKALNNAIAVLKEKKEELLSEQIDNO:14WP_061396064.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKSLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLGKYKLSFPAQEFELYCLIKEFESANIEVLTFTFNRFENEEHADIEKDVKKALNNAIAVLKEKKEELLSEQIDNO:15WP_014930689.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKSLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLGKYKLSFPAQEFELYCLIKEFESANIEVLTFTFNRFENEEHADIEKDVKKALNNAIAVLKAKKEELLSEQIDNO:16WP_061105218.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKSLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLGKYKLSFPAQEFELYCLIKEFESANIEVLTFTFNRFENEEHADIEKDVKKTLNNAIAVLKEKKEELLSEQIDNO:17WP_070216262.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLTFTFNRFENEEHADIEKDVKKALNNAIAVLKEKSEQIDNO:18WP_070761486.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKSLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLGKYKLSFPAQEFELYCLIKEFESANIEVLTFTFNRFENEEHADIEKDVKKALNNAIAVLKEKKKNCYKNYSEQIDNO:19WP_070005110.1MSIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKSLNKYTVSILRALALITGMPISDVLFELEDLEKNADDLAGFKHLLDTYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFESETHVDIEKDVRKALENAITVLKEKKNEFMSEQIDNO:20WP_070784182.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNIEKDVCKASEQIDNO:21WP_070776459.1MSIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKTLNKYTVSILRALALITGMPISDVLFELEDLEKNADDLAGFKHLLDTYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFESETHVDIEKDVQKALENAITVLKEKKNEFMSEQIDNO:22WP_003737351.1MSIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKTLNKYTVSILRALALITGMPISDVLFELEDLEKNADDLAGFKQLLDTHKLSFPAHEFELYCLIKEFESVNIEVLPFTFNRFESETHVDIEKDVRKALENAITVLKEKKNEFMSEQIDNO:23WP_010991654.1MSIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKTLNKYTVSILRALALITGMPISDVLFELEDLEKNADDLAGFKQLLDTHKLSFPAHEFELYCLIKEFESVNIEVLPFTFNRFESETHVDIEKDVRKALENAITVLKEKKNEFISEQIDNO:24WP_070295945.1MSIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKTLNKYTVSILRALALITGMPISDVLFELEDLEKNADDLAGFKQLLDTHKLSFPAHEFELYCLIKEFESVNIEVLPFTFNRFESETHVDIEKDVQKALENAIAVLKEKKEELLSEQIDNO:25WP_061662200.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKPLNKYTVSILRSLSLISGLSVSDVLFELEDIEKNSDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNSEQIDNO:26WP_061665494.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNIKKDVCKALENAITVLKEKKNELLSEQIDNO:27WP_061107167.1MSIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNIKKDVCKALENAITVLKEKKNELLSEQIDNO:28WP_070005290.1MSIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNIEKDVCKALENAITVLKEKKNELLSEQIDNO:29WP_039385152.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCTGISTFDVFIELAELEKSYDDLAGFKHLLNKYKLSFPAQEFELYCLIKEFDSANIEVLPFTFNRFENEEHVNIEKDVCKALENAITVLKEKKNELLSEQIDNO:30WP_015967154.1MSIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNIEKDVCKALENAITVLKEKKNELISEQIDNO:31WP_069001242.1MNIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCTGISTFDVFIELAELEKSHDDLAGFKHLLDKHKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNIEKDVCKALENAITVLKEKKNELLSEQIDNO:32WP_070040173.1MSIKLLDEFLKKHDLTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCTGISTFDVLIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:33WP_068999238.1MTIKLLDEFLKKHSLTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVLIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:34WP_070784648.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVLIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:35WP_070777825.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVLIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFECANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:36WP_047934326.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:37WP_003727802.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCTGISTFDVFIELAELEKNYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFECANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:38WP_003723291.1LMOG_02992MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFNELEELEKNYDDLAGFKHLLDKYKLSFSAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:39WP_069027465.1MTIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFECANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:40WP_047933338.1MSIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:41WP_060579665.1MSIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFECANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELLSEQIDNO:42WP_031646274.1MTIKLLDEFLKKHDLTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCTGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHTDIEKDVKKTLNNAIAVLKEKKEELLSEQIDNO:43WP_070776287.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFECANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:44WP_031645842.1MSIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFMRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:45WP_046376633.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFKELEELEKNYDDLAGFKHLLDKYKLSFSAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:46WP_070242402.1MSIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLSGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:47WP_047923954.1MSIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESASIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:48WP_031668927.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCTGISTFDVFIELAELEKSHDDLAGFKHLLDKHKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:49WP_012581438.1MSIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFECANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:50WP_031667947.1MSIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFECANIEVLPFTFNRFENETHVDIEKDIRKALENAITVLKEKKNELISEQIDNO:51WP_061107116.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYTVSFLRTLSMCVGMSTVDVFIELAELEKNYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFESETHVDIEKDVKKALNNAIAVLKEKKEELLSEQIDNO:52WP_070783481.1MSIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALNNAIAVLKEKKEELLSEQIDNO:53WP_060577773.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKYLLDKHKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:54WP_039389295.1MNIKLLDEFLKKHDLTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCTGISTFDVFIELAELEKSYDDLAGFKHLLDKHKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHADIEKDVKKALNNAIAVLKAKKEELLSEQIDNO:55WP_070295880.1MSIKLLDEFLKKHDLTRYQLSKLTGISQNTLKDQNEKTLNKYTVSILRALALITGMPISDVLFELEDLEKNADDLAGFKQLLDTHKLSFPAHEFELYCLIKEFESVNIEVLPFTFNRFESETHVDIEKDVSEQIDNO:56WP_061399219.1TRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNIKKDVCKALENAITVLKEKKNELLSEQIDNO:57WP_061112070.1MKINLLDEFLKRHNITRYRLSKLAGISQNTLKDYNEKSLNKYTVSFLRSLSFVTGEDVTDVLIELAELEKGYDDLAGFKYLLDKYKLSFPALEFELYCIIKEFESANIEISPFTFNRFENETHVDIEKDVKKALQNAVTVLEERKEELLSEQIDNO:58WP_070779352.1MKNNLLDTFLKRHDITRYRLSKLAGISQNTLKDYNEKSLNKYTVSLLRSLSFVTGESITDVLLELAEIEKDYDDLAGFKYLLDKYKLSFPALEFELYCIIKEFESANVEISPFTFNRFENETHADIEKDVKKALNNAITVLKEKKEELLSEQIDNO:59WP_014930929.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKYLLDKHKLSFPTQEFELYCLIKEFESANIEVLPFTFNRFENETHADIEKDVKKALNNAIAVLEEKKEELLSEQIDNO:60WP_069001897.1MKINLLDAFLKRHNITRYRLSKLAGISQNTLKDYNEKSLNKYTVSFLRSLSFVTGEDVTDVLIELAELEKGYDDLAGFKYLLDKYKLAFPALEFELYCLIKEFEAANIEVSPFTFNRFENETHADIEKDVKKALKNAIIVLKEKKEELLSEQIDNO:61WP_070784143.1MSIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNIEKDVCKASEQIDNO:62EFS02359.1MKINLLDEFLKRHNITRYRLSKLAGISQNTLKDYTEKSLNKYTVSFLRSLSFATGESVTDILLELAELEKDYDDLAGFKYLLDKYKLAFPALEFELYCLIKEFESANIEISPFTFNRFESETHTDIEKDVKKALQNAVTVLEERKEELLSEQIDNO:63WP_061128861.1MSIKLLDEFLKKHSKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRSLSFATGESVTDILLELAELEKDYDDLAGFKYLLDKYKLAFPALEFELYCLIKEFESANIEISPFTFNRFESETHTDIEKDVKKALQNAVTVLEERKEELLSEQIDNO:64KUG37233.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKYLLDKHKLSFPTQEFELYCLIKEFESANIEVLPFTFNRFENETHADIEKDVKKALNNAIAVLEEKKRRTVIKTIDYYDYSSEQIDNO:65WP_049955951.1MNNFAFITSFNYQQPRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVLIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:66WP_009917642.1MKTNLLDTFLKRHGITRYRLSKLAGISQNTLKDYTEKSLNKYTVSFLRSLSFVTGEDVTDVLLELAEIENGYDDLAGFKYLLDKYKLSFPALEFELYCIIKEFESANIEISPFTFNRFENETHADIEKDVKKALKNAVTVLEERKEELLSEQIDNO:67WP_070777879.1MNNFAFITSFNYQQPRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVLIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFECANIEVLPFTFNRFENETHVDIEKDVRKALENAITVLKEKKNELISEQIDNO:68WP_061662201.1MSIKLLDEFLKKHNKTRYQLSKLTGISQNTLNDYNKKELNKYSVSFLRALSMCAGISTFDVFIELAELEKSYDDLAGFKHLLDKYKLSFPAQEFELYCLIKEFESANIEVLPFTFNRFENEEHVNAcrIIA2蛋白序列SEQIDNO:69WP_003722517.1MTLTRAQKKYAEAMHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETNDIDDLEIATDEDEMKSGNQEIILKSELKSEQIDNO:70WP_031668925.1MTLTRAQKKYAEAMHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETNDIDDLEIATDEDEMKSGNQEIILKSELNSEQIDNO:71WP_031646276.1MTITRAQKKYAEAMHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETNDIDDLEIATDEDEMKSGNQEIILKSELKSEQIDNO:72EZH71062.1KMTLTRAQKKYAEAMHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETNDIDDLEIATDEDEMKSGNQEIILKSELNSEQIDNO:73WP_061662199.1MTLTRAQKKYAEAMHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETNDIDDLEIATDEDEMKSGNQEIILKSELSEQIDNO:74WP_070026783.1MTLTRAQKKYAEAMHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETNDIDDLEIATDEDEMKSDNQEIILKSELKSEQIDNO:75WP_068996202.1MTITRAQKKYAEAMHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDGCEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETNDIDDLEIATDEDEMKSGNQEIILKSELKSEQIDNO:76WP_009928183.1MTLTRAQKKYAEAMHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETKDIDDLEIATDEDEMKSDNQEIILKSELKSEQIDNO:77WP_014930690.1MTITTAQKKYAEAMHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETNDIDDLEIATDEDEMKSGNQEIILKSELKSEQIDNO:78WP_061394923.1MTITTAQRKYNEAMHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETNDIDDLEIATDEDEMKSGNQEIILKSELKSEQIDNO:79WP_069001241.1MTITTAQRKYNEAMHEFINMVDDFEESTPDFAKEVLHDCDYVVVTKNEKYAVALCTLSTDECEYDTNLYLDEKLVDYSTVNVNGVTYYINIVETNDIDDLEIATDEDEMKSDNQEIILKSELKSEQIDNO:80WP_039389299.1MTITTAQRKYNEAMHEFINMVDDFEESTPDFAKEVLHDCDYVVVTKNEKYAVALCTLSTDECEYDTNLYLDEKLVDYSTVNVNGVTYYINIVETNDIDDLEIATDEDEMKSDNQKIILKSELKSEQIDNO:81WP_039385155.1MTLTRAQKKYAEAMHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETKDIDDLEIATDEDKEKHDKQEVIIKSELNSEQIDNO:82WP_003733874.1MHEFINMVDDFEESTPDFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVETNDIDDLEIATDEDEMKSGNQEIILKSELKSEQIDNO:83WP_070294198.1MTLTRAQKKYAEAMHEFINMVDDFEESKPNFAKEVLHDSDYVVITKNEKYAVALCSLSTDECEYDTNLYLDEKLVDYSTVDVNGVTYYINIVVTNEDDFKLATDKDKEKHDKQEVIVKSELNSEQIDNO:84WP_031649390.1MTLTTAQRKYNEAMHEFINMVDDFEESTPEFSKEVLNDSDYVVITKNEKYAGALCHVSTDECEDGSNLYIDEKLIDYSTLNVGGVTYYINIVERCEDDLEIATDEDKMKSDNQEIILKNELNSEQIDNO:85EFR93689.1MVDDFEESTPEFSKEVLNDSDYVVITKNEKYAGALCHVSTDECEDGSNLYIDEKLIDYSTLNVGGVTYYINIVERCEDDLEIATDEDKMKSDNQEIILKNELNSEQIDNO:86WP_070295879.1MTLTTAQKRYYDAMNEFEAITSKKLEQTPEFSQDLLNDSDYLVITKNEAYAVALCMLDDDKLYLDETLVQSTCLDVEGETYYINFVVTNEDDFKLATDEDKEKHDKQEVIVKSELNSEQIDNO:87WP_070776458.1MTLTTAQKRYYDAMNEFEAITSKELEQTPEFSQDLLNDSDYLVITKNEAYAVALCMLDDDKLYLDETLVQSTCLDVEGETYYINFVVTNEDDFKLATDEDKEKHDKQEVIVKSELNSEQIDNO:88WP_070005111.1MTLTTAQKRYYDAMNEFEAITSKELEQTPEFSQDLLNDSDYLVITKNEAYAVALCMLDDDKLYLDETLVQSTCLDVEGETYYINFVVTNEDDFKLATDKDKEKHDKQEVIVKSELNSEQIDNO:89WP_023553814.1MTITTAQKRYYDAMNEFEAIISKELEQTPAFSQDLLNDSDYLVITKNEAYAVALCMLDDDKLYLDETLVQSTRLDIEDETYYINFVVTNEDDFKLATDEDKEKHDKQEVIIKSELNSEQIDNO:90WP_031645843.1MTITTAQKRYYDAMNEFEAITSKELEQTPEFSQDLLNDSDYLVITKNEAYAVALCMLDDDKLYIDETLVQSTCLDVEGETYYINFVVTNEDDFKLATDKDKEKHDKQEVIIKSELNSEQIDNO:91WP_014930930.1MTLTTAQKRYYDAMNEFEAIISKELEQTRAFSQDLLNDSDYLVITKNEAYAVDLCMLDDDKLYLDETLVQSTRLDIEDETYYINFVVTNEDDFKLATDEDKEKHDRQEVIIKSELNSEQIDNO:92WP_070783480.1MTITTAQKRYYDAMNEFEAITSKELEQTPEFSQDLLNDSDYLVVTKNEAYAAALCMLDDDKLYLDETLVQSTCLDVEGEIYYINFVVTNEDDFKLATDKDKEKHDKQEVIVKSELNSEQIDNO:93WP_070040172.1MTITTAQKRYYDAMNEFEAITSKGLEQTPEFSQDLLNDFDYLVITKNEAYAAALCMLDDEKLYLDETLVHSTRLDIEDDTYYINFVVTNEDDFKLATDEDKEKHDKQEVIIKRELNSEQIDNO:94WP_012581437.1MTITTAQKRYYDAMNEFEAIISKELEQTPAFSQDLLNDSDYLVITKNEAYAVALCLLDDDKLYLDETLVHSTRLDIEDETYYINFVVTNEDDFKLATDEDKEKHDKQEVIIKSELNSEQIDNO:95WP_061107168.1MTITTAQKRYYDAMNEFEAIISKELEQTPAFSQNLLNDSDYLVITKNEAYAVALCLLDDDKLYLDETLVHSTRLDIEDETYYINFVVTNEDDFKLATDEDKEKHDKQEVIIKSELNSEQIDNO:96WP_070243058.1MTITTAQKRYYDAMNEFEAITSKELEQTPAFSQDLLNDSDYLVITKNEAYAVALCLLDDDKLYLDETLVHSTRLDIEDETYYINFVVTNEDDFKLATDEDKEKHDKQEVIVKSELNSEQIDNO:97WP_015967155.1MTITTAQKRYYDAMNEFEAIISKELEQTPAFSQDLLNDSDYLVITKNEAYAVALCLLDDDKLYLDETLVHSTRLNIEDETYYINFVVTNEDDFKLATDEDKEKHDKQEVIIKSEFNSEQIDNO:98WP_010989941.1MTLTTAQKRYYDAMNEFEAITSKELEQTPEFSQDLLNDSDYLAVTKNEAYAVALCLLDDDKLYLDETLVHSTRLDIEDETYYINFVVTNEDDFKLATDEDKEKHDKQEVIIKSGLNSEQIDNO:99WP_010991653.1MTVTTAQKRYYDAMNEFEAITSKELEQTPEFSQDLLNDSDYLAVTKNEAYAVALCLLDDDKLYLDETLVHSTRLDIEDETYYINFVVTNEDDFKLATDEDKEKHDKQEVIIKSELNSEQIDNO:100WP_068996515.1MTLTTVQKRYYDAMNEFEAITSKELEQTPEFSQDLLNDSDYLAVTKNEAYAVALCLLDDDKLYLDETLVHSTRFDIEDETYYINFVVTNEDDFKLATDEDKEKHDKQEVIIKSELNSEQIDNO:101WP_047934322.1MTLTTVQKRYYDAMNEFEAITSKELEQTPEFSQDSLNDSDYLAVTKNEAYAVALCLLDDDKLYLDETLVHSTRFDIEDETYYINFVVTNEDDFKLATDEDKEKHDKQEVIIKSELNSEQIDNO:102WP_003727803.1MTITTAQKRYYDAMNEFEAIISKELEQTPAFSQDLLNDSDYLAVTKNEAYAVALCLLDDDKLYLDETLVHSTRLDIEDETYYINFVVTNEDDFKLATDEDKEKHDKQEVIIKSGLNSEQIDNO:103WP_061114505.1MTITTAQKRYYDAMNEFEAITSKELEQTPAFSQDLLNDSDYLAVTKNEAYAVALCLLDDDKLYLDETLVHSTRLDIEDETYYINFVVTNEDDFKLATDEDKEKHDKQEVIIKSGLNSEQIDNO:104EFS02358.1MAITLSQRKFYEAINEFEEMTENEVVTSPRIPQDYLNDGDYVVITKSENYALNLCTTNLEGFEDRHFLDEKLIYSTFVETYSGETYYIYITQTAEFDEDDAVEFLATQEQIYEYHKQEEQKTVILKMELSSEQIDNO:105WP_069001896.1MAQTEAQKIFYEAINEFEEMTNEEVVTSPRIPQDYLNDGDYVVITKSENYALNLCTTDLEGFEDRYFLDEKLIYSTSVETYTDETYYIYITQTTEFEEDNAVEFLATQEQIYEYHKQEEQKTVILKMELSSEQIDNO:106WP_061665680.1MTTARKKFYQAISEFEAMTGKDVERTPQIADEVLNDAEYIAFTKTEKYALYLCTSNVEGLEDRYFLDEECLDSTFLETEDNETYYIHFLQETEFSEDDNEDELPLATEEQIEAYDKQEELKAVILKKELNSEQIDNO:107WP_009917643.1MRTTAQERLDNAINEFEEITNEEVVTSPRIPQDYLNDGDYVVITKSENYALNLCTTNLEGFEDRHFLDEKLIYSTFVETYSGETYYIYITQTAEFDEDDAVEFLATQEQIYEYHKQEEQKTVILKMELSSEQIDNO:108WP_061112069.1MRTTAQERLDNAINEFEEITNEEVVTSPRIPQDYLNDGDYVVITKSENYALNLCTTNLEGFEDRHFLDEKLIYSTFVETYAGETYYIYITQTAEFDEDDAVEFLATQEQIYEYHKQEEQKTVILKMELSSEQIDNO:109WP_003745974.1MRTTAQERLDNAINEFEEITNEEVVTSPLIPQDYLNDGDYVVITKSENYALNLCTTNLEGFEDRHFLDEKLIYSTFVETYSGETYYIYITQTAEFDEDDAVEFLATQEQIYEYHKQEEQKTVILKMELSAcrIIA3蛋白序列SEQIDNO:110WP_014930691.1李斯特菌MFNKAEIMKQAWNWFNDSNIWLSDIEWVSYTDKEKSFSVCLKAAWSKAKEEVEESKKESKHIAKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:111WP_068996201.1MYNKAEIMKQAWNWFNDSNIWLSDIEWVSYTDKEKSFSVCLKAAWSKAKEEVEESKKESKHIAKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:112WP_031646277.1MYNKAEIMKQAWNWFNDSNIWLSDIEWVSYTDKEKSFSVCLKGAWSKAKEEVEESKKESKHIAKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:113WP_003727804.1MYNKAEIMKQAWNWFNDSNIWLSDIEWVSYTDKEKSFSVCLKAAWSKAKEEVEESKKESKHIAKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSLWACAMKAVKLHNDLFPQTAASEQIDNO:114WP_070776457.1MYNKAEIMKQAWNWFNDSNVWLSDIEWISYTDKEKTFSVCLKAAWSKAKEEVEESKKESKHIAKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:115WP_015455142.1MYNKAEIMKQAWNWFNDSNIWLSDIEWVSYTDKEKSFSVCLKAAWSKAKEEVEESKKESKYIAKSEELKAWNWAERKLGLRFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:116WP_070005112.1MFNKAEIMKQAWNWFTDSNVWLSDIEWASYTDKEKSFSVCLKAAWSKAKEEVEESKKESKHIAKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:117WP_068996392.1MYNKAEIMKQAWNWFNDSNIWLSDIEWVSYTDKEKSFSVCLKAAWSKAKEEVEEFKKESKYIAKSEELKAWNWAERKLGLRFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:118CUK89695.1MKQAWNWFNDSNIWLSDIEWVSYTDKEKSFSVCLKAAWSKAKEEVEESKKESKYIAKSEELKAWNWAERKLGLRFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:119WP_060577772.1MYNKAEIMKQAWNWFNNSNVWLSDIEWVSYTDKEKTFSVCLKAAWSKAKEEVEESKEESKHIAKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:120WP_070295878.1MYNKAEIMKQAWNWFNNSNVWLSDIEWVSYTDKEKTFSVCLRAAWSKAKEEVEESKEESKHIAKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:121WP_061396065.1MFNKAEIMKQAWNWFTDSNVWLSDIEWVSYTDKEKTFSVCLKAAWSKAKEEVKEVEKEIKHISKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:122WP_039389302.1MYNKAEIMKQAWNWFNDSNVWLSDIEWASYTDKEKTFSVCLKAAWSKAKEEVKEVEKEIKHISKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:123WP_047934319.1MFNKAEIMKQAWNWFTDSNVWLSDIEWASYTDKEKTFSVCLKAAWSKAKEEVKEVEKEIKHISKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:124WP_069001240.1MYNKAEIMKQAWNWFTDSNVWLSDIEWASYTDKEKTFSVCLKAAWSKAKEEVKEVEKEIKHISKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:125WP_010991652.1MFNKAEIMKQAWNWFTDSNVWLSDIEWVSYTDKEKTFSVCLKAAWSKAKEEFKEVEKEIKHISKSEELKAWNWAERKLGLHFNISDDEKFTSVKNETKMNFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:126WP_061114504.1MYNKAEIMKQAWNWFTDSNVWLSDIEWASYTDKEKTFSVCLKAAWSKAKEEVKEVEKEIKHISKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTVASEQIDNO:127WP_014930931.1MYNKAEIMKQAWNWFNDSNVWLSDIEWASYTDKEKTFSVCLKAAWSKAKEEVKEVEKEIKHISKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHSHLFPQTAASEQIDNO:128WP_069002681.1MYNKAEIMKQAWNWFTDSNVWLSDIEWASYTDKEKTFSVCLKAAWSKAKEEVKEVEKEIKHISKREELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTVASEQIDNO:129WP_012581436.1MFNKAEIMKQAWNWFTDSNVWLSDIEWVSYTDKEKTFSVCLKAAWSKAKEEVKEVEKEIKHISKSEELKAWNWAERKLGLRFNISDDEKFTSVKDETKQHFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:130WP_010989940.1MYNKAEIMKQAWNWFNDSNIWLSDIEWVSYTDKEKSFSVCLKAAWSKAKEEVEESKKESKHIAKSEELKAWNWAEIKLGLRFNISDDEKFTSVKDETKMNFDLNVWACAMKAVKLHNDLFPQTAASEQIDNO:131WP_015967156.1MYNKAEIMKQAWNWFTDSNVWLSDIEWVSYTDKEKTFSVCLKAAWSKAKEEVKEVEKEIKHISKSEELKAWNWAERKLGLRFNISDDEKFTSVKDETKQHFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:132WP_031645844.1MYNKAEIMKQAWNCFNDSNVWLSDIEWVSYTDKEKTFSVCLKAAWSKAKEEIEKSKKESKHIAKSEELKAWNWAERKLGLRFNISDDEKFTSVKDETKQHFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:133WP_031691597.1MYNKAEIMKQAWNCFNDSNVWLSDIEWVSYTDKEKTFSVCLKAAWSKAKEEIEESKKESKHIAKSEELKAWNWAERKLGLRFNISDDEKFTSVKDETKQHFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:134WP_023553812.1MYNKSEIMQQAWNWFRDSSVWLSDIEWVSYTDKEKTFSVCLKAAWSKAKEEVEESKKESKHIAKSEELKAWNWAESKLGLRFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTAASEQIDNO:135WP_069029656.1MYNKAEIMKQAWNWFTDSNVWLSDIEWASYTDKEKTFSVCLKAAWSKAKEEVKEVEKEIKHISKREELKAWNLAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNDLFPQTVASEQIDNO:136WP_069001927.1MFNKAEIMKQAWNWFTDSNVWLSDIEWASYTDKEKTFSVCLKAAWSKAKEEVKEVEKEIKHISKSEELKAWNWAERKLGLHFNISDDEKFTSVKDETKINFGLSVWACAMKAVKLHNSEQIDNO:137WP_064659125.1MKKVTYDKSGIMKEAWNLFNNDDITLADFEHLGWMEWKSEKTFALCLKEAWGREKEVVERVNQKFANAETSEEAKAWDWACKKLGVAFEMDAYTKMTNVEDMEKEAWPGTSVWSLAMRAVKLHMEVAASEQIDNO:138WP_012678885.1MRYNKSEIMKNAWAMFNSCNWGAENFKFVSVEEKTFAACLKEAWAEEKEYVEEKIKESANAPKSEEAKAWDWACRKLNANKLQNVEATDKVAWVSEMAKEMWSSNIWAQAIKAVKLHIKLFAASEQIDNO:139WP_037595340.1MKYNKSEIMKNAWTMFNDDNFDTSYYEYATAEVYGQKTFSECLKESWGREKAYQEEKEKRLVDAPKSEEAKAWDWACRKLNVNELQNIDATDKVFYVEGMAKEMWSSNVWAQAIKAVKLHIELFVASEQIDNO:140WP_009730539.1MKKVAYDKSGIMKEAWEMFNRNYQICDFEYADFSGREYFEYASFADCLKEAWAHEKEVVERVNQKYADAETSEEVKAWDWACKKLGVAFEMDAYTKITNVEGMEKEAWPGTSVWSLAMRAVKLHMEVAASEQIDNO:141WP_071127625.1MAKYNKSEIMTQAWTLFNSDNFDTCDYEYTTALVYGQKNFSDCLKEAWGREKAIVERMAEQEANAPLSEEAKAWDWACRKLGVTAEVTAVEKVRYVDDMAKEMWSANVWKQAIKAVQLYATVASEQIDNO:142AGM98706.1MAKYNKSEIMTQAWTLFNSDNFDTCDYEYATALVYGQKTFSDCLKEAWGREKAIVERMAEKEANAPLSEEAKAWDWACRKLGVTAEVTAVEKVRYVDDMAKEMWSTNVWKQAIKAVQLYATVASEQIDNO:143WP_012767357.1MAKYNKSEIMKNAWAMFNSYEWDVENFKFVSAENKTFSNCLKEAWAEEKEYVERKAKETAEAPKSEEAKAWDWACRKLNVNDLQNIDATDKVFYVVDMQKEMWTSNVWAQAIKAVELYVKLGLASEQIDNO:144WP_023611744.1链球菌MTKYNKSEIMKNAWAMFNSYEWDVENFKFVSAENKTFSNCLKEAWAEEKEYVERKAKETAEAPRSEEAKAWDWACRKLNVNDLQNIDATDKVFYVVDMQKEMWTSNVWAQAIKAVELYVKLGLASEQIDNO:145WP_066028552.1MTKYNKSEIMKNAWAMFNSYEWDVENFKFVSAENKTFSNCLKEAWAEEKEYVERKAKETAEAPKSEEAKAWDWACRKLNVNDLQNIDATDKVFYVVDMQKEMWTSNIWAQAIKAVELYVKLGLASEQIDNO:146WP_003055844.1MTKYNKSEIMKNAWAMFNSYEWDVENFKFVSAENKTFSNCLKEAWAEEKEYVERKAKEAAEASKSEEAKAWDWACRKLNVNDLQNIDATNKVFYVVDMQKEMWTSNVWAQAIKAVELYVKLGLAAcrIIA4蛋白序列SEQIDNO:147WP_003723290.1MNINDLIREIKNKDYTVKLSGTDSNSITQLIIRVNNDGNEYVISESENESIVEKFISAFKNGWNQEYEDEEEFYNDMQTITLKSELNSEQIDNO:148WP_046376634.1MNINELIREIKNKDYTAKLSGTDSNSITQLIIRVNNDGNEYVISESENESIVEKFISAFKNGWNQEYEDEEEFYNDMQTITLKSELNSEQIDNO:149WP_069001216.1MNINELIREVKNKDYTAKLSGTDSNSITQLIIRVNNDGNEYVISESENESIVEKFISAFKNGWNQEYEDEEEFYNDMQTITLKSELNSEQIDNO:150KLI10194.1MNINELIREIKNKDYTAKLSGTDSNSIAQLIIRVNNDGNEYVISESENESIVEKFISAFKNGWNQEYEDEEEFYNDMQTITLKSELNSEQIDNO:151WP_031667946.1MNINELIREIKNKDYTAKLSGTDSNSITQLIIHVNNDGNEYVISESENESIVEKFISAFKNGWNQEYEDEEEFYNDMQTITLKSELNSEQIDNO:152WP_070295973.1MNINDLIREIKNKDYTVKLSGTDSNSITQLIINVNNDGNEYGISESNFESIVEKFVSNFENGWDGAYEDEEEFYNDMQAIILKSELNSEQIDNO:153WP_061107115.1MNINDLIREIKNKDYTVKLSGTDSNSITQLIINVNNDGNEYGISESNFESIVEKFVSNFENGWDGAYEDEEEFYNDMQAIILKSESNSEQIDNO:154WP_060954847.1MNISELIREIKNKDYTVRLEGTDDNSITKLIIDVDNDGNEYVISESKNESIAEKFASTFKNGWNKEYEDEEEFYNDMQSIILKSELNSEQIDNO:155WP_012582157.1MNISELIREIKNKDYAVRLEGTDDNSITKLIIDVDNDGNEYVISESKNESIAEKFASTFKNGWNKEYEDEEEFYNDMQSIILKSELNSEQIDNO:156CAR82813.1MAGYLKRYAEDRGWTLYRLAKESHLSDSTLRTADLTTLNKLSVINIKKISEAVGETPGEVLDDLIKFEERVEKMNISELIREIKNKDYAVRLEGTDDNSITKLIIDVDNDGNEYVISESKNESIAEKFASTFKNGWNKEYEDEEEFYNDMQSIILKSELNSEQIDNO:157WP_003740262.1MNLKELVREIKNKDYTAKLSGTDSNSITQLIIHVNNDGNEYGISESNFESIVEKFVSTFENGWDGAYEDEEEFYNDMQDIVNRHFKSEQIDNO:158WP_061385557.1MNLKELVREIKNKDYTAKLSGTDSNSITQLIIHVNNDGNEYGISESNFESIVEKFVSNFENGWDGAYEDEEEFYNDMQDIVNRHFKSEQIDNO:159CUL91420.1MKINELVREIKSRDYTVRLNGTDSNSITKLIIDVNNDGNEYVISERQDTSIVESFADSFIDGWTGTYEDEEDFYNDMQEIAQDIILETLKEAFENNNYNTDEVDTDLFDGYQIKLAMEYDNIGELATSVNKTKHFTAYMDASTDFMIIEKYSEQIDNO:160YP_008239985.1MSIIAIKKEIHAKGYKVTGTHQGYIAQINFDGTGNEYPLPATWDEFIETFKDGWNGTYEDEQAFFNDMQEVALKEILDELTGALFCQDITTYDFTIDDVKKKVITLDKPTFEEDAEDLIIEFDSTCFWDATVENDKIKITVRNKSRYSEQIDNO:161AII27415.1MSIIAIKKEIHAKGYKVTGTHQGYIAQINFDGTGNEYPLPATWDEFIETFKDGWNGTYEDEQAFFNDMQEIALDEILDELIDVLYNLDITTYNFTIDDSSEQIDNO:162YP_001468568.1MSIIAIKKEIHAKGYKVTGTHQGYIAQINFDGTGNEYPLPATWDEFIETFKDGWNGTYEDEQAFFNDMQEIALEEILDELTGALFCQDITTYDFTIDDVKKKVITLDKPTFEEDAEDLIIEFDSTCFWDATVENDKIKITVRNKSRYSEQIDNO:163YP_009043548.1MSIIAINKEIRAKGYKVTGTHQGYIAQINFEGTGNEYPLPATWDEFIETFKDGWNGTYEDEQAFFNDMQEIALDEILDELIDVLYNLDITTYNFTIDDVKKKVITLNKPIDEEETEDLVQEFNVTCFWDATVEDDKVKVTIRNKNRAISSEQIDNO:164AID17477.1MSTTAINKEIHAKGYKVTGTHQGYIAQINFDGTGNEYPLPATWEKFIETFKDGWDGTYEDEQAFFNDMQEIALDEILDELIDVLYNLDITTYNFTIDDVKKKVITLNKPIDEEETEDLVQEFNVTCFWNAIVEDDKVKITVRNKSKSEQIDNO:165YP_009043010.1MSTTAINKEIHAKGYKVTGTHQGYIAQINFDGTGNEYPLPATWEEFIETFKDGWDGTYEDEQAFFNDMQEIALDEILDELIDVLYNLDITTYNFTIDDVKKKVITLNKPIDEEETEDLVQEFNVTCFWNAIVEDDKVKITVRNKSKSEQIDNO:166AID17274.1MSTTAINKEIHAKGYKVTGTHQGYVAQINFDGTGNEYPLPATWEEFIETFKDGWDGTYKDEQAFFNDMQEIALDEILDELIDTLYNLDITTYDFTIDDIKKKVITLDKPTDREETEDLVQEFNVTCFWNAIVEDDKVKVTVRNKSKSEQIDNO:167AAY53411.1MTGTHQGYIAQINFDGTGNEYPLPATWDEFIETFKDGWNGTYEDEQAFFNDMQEVALKEILDELIDVLYNLDITTYNFTIDDVKKKVITLNKPTDEEDAEDLVIEFDSTCFXDATVENDKIKVTVRNKSKSEQIDNO:168YP_009044467.1MSTTAINKEIHAKGYKVTGTHQGYMAQINFDGTGNEFPLPATWEEFIETFKDGWDGTYEDEQAFFNDMQEVALEELLDELTDVFYNLDITAYDFTVDDVKKKVITLDKPTDREETEDLVQEFKATCFWNAVVEDDKVKVTIRNKNRAIS其它蛋白质序列SEQIDNO:169AcrIIA3b.3,OLF47316.1MQFVVTNKSELFKFAWKIFKANKDIAFSECLQNAWFQYKRYLNREAIKAAQQRKLAKFIADTENEEVKAWNWAEKKLGVALNLTDAEKERNVRNMYKEMWNANVWATAIKAVKLHMEIGSEQIDNO:170AcrIIA4b.2,YP008240385.1MNELRSLEMSINAKKYDTRLESGNRVLNIGFGDGEDYPVCSSSRYSLKESFIECFKDGWSGTYRDEKELMEDMQEIAQELILEELTDIFEYYEFNTDEIDTDLFKGFTFDVDSDLEDSMALMKAINATKYFEARSSSWYASFEVSYIG序列表加利福尼亚大学董事会TheRegentsoftheUniversityofCaliforniaJ·邦迪-蒂诺米Bondy-Denomy,JosephB·劳奇Rauch,BenjaminCRISPR-Cas9抑制剂1064235US62422,8502016-11-16209PatentInversion3.51149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽1MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsnIleLysLys115120125AspValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuLeu1452149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽2MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerThrAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsnIleLysLys115120125AspValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuLeu1453148PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽3MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAsnIleGluValLeuProPhe100105110ThrPheAsnArgPheGluAsnGluGluHisValAsnIleGlyLysAsp115120125ValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLysLys130135140AsnGluLeuLeu1454149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽4MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsnIleGluLys115120125AspValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuLeu1455148PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽5MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAsnIleGluValLeuProPhe100105110ThrPheAsnArgPheGluAsnGluLysHisValAsnIleLysLysAsp115120125ValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLysLys130135140AsnGluLeuLeu1456149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽6MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAsnLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsnIleGluLys115120125AspValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuLeu1457149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽7MetAlaIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluLysHisValAsnIleLysLys115120125AspValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuLeu1458149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽8MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsnIleGluLys115120125AspValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuLeu1459147PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽9MetLysLeuLeuAspGluPheLeuLysLysHisAspLeuThrArgTyr151015GlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLysAspGln202530AsnGluLysProLeuAsnLysTyrThrValSerIleLeuArgSerLeu354045SerLeuIleSerGlyLeuSerValSerAspValLeuPheGluLeuGlu505560AspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHisLeuLeu65707580AspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeuTyrCys859095LeuIleLysGluPheGluSerAlaAsnIleGluValLeuProPheThr100105110PheAsnArgPheGluAsnGluLysHisValAsnIleLysLysAspVal115120125CysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLysLysAsn130135140GluLeuLeu14510149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽10MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAsnLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuLeu14511149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽11MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14512149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽12MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysSerLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuGlyLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14513149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽13MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuThr100105110PheThrPheAsnArgPheGluAsnGluGluHisAlaAspIleGluLys115120125AspValLysLysAlaLeuAsnAsnAlaIleAlaValLeuLysGluLys130135140LysGluGluLeuLeu14514149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽14MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysSerLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuGlyLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuThr100105110PheThrPheAsnArgPheGluAsnGluGluHisAlaAspIleGluLys115120125AspValLysLysAlaLeuAsnAsnAlaIleAlaValLeuLysGluLys130135140LysGluGluLeuLeu14515149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽15MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysSerLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuGlyLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuThr100105110PheThrPheAsnArgPheGluAsnGluGluHisAlaAspIleGluLys115120125AspValLysLysAlaLeuAsnAsnAlaIleAlaValLeuLysAlaLys130135140LysGluGluLeuLeu14516149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽16MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysSerLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuGlyLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuThr100105110PheThrPheAsnArgPheGluAsnGluGluHisAlaAspIleGluLys115120125AspValLysLysThrLeuAsnAsnAlaIleAlaValLeuLysGluLys130135140LysGluGluLeuLeu14517144PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽17MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuThr100105110PheThrPheAsnArgPheGluAsnGluGluHisAlaAspIleGluLys115120125AspValLysLysAlaLeuAsnAsnAlaIleAlaValLeuLysGluLys13013514018152PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽18MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysSerLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuGlyLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuThr100105110PheThrPheAsnArgPheGluAsnGluGluHisAlaAspIleGluLys115120125AspValLysLysAlaLeuAsnAsnAlaIleAlaValLeuLysGluLys130135140LysLysAsnCysTyrLysAsnTyr14515019149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽19MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysSerLeuAsnLysTyrThrValSerIleLeuArg354045AlaLeuAlaLeuIleThrGlyMetProIleSerAspValLeuPheGlu505560LeuGluAspLeuGluLysAsnAlaAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspThrTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluSerGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluPheMet14520133PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽20MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsnIleGluLys115120125AspValCysLysAla13021149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽21MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysThrLeuAsnLysTyrThrValSerIleLeuArg354045AlaLeuAlaLeuIleThrGlyMetProIleSerAspValLeuPheGlu505560LeuGluAspLeuGluLysAsnAlaAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspThrTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluSerGluThrHisValAspIleGluLys115120125AspValGlnLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluPheMet14522149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽22MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysThrLeuAsnLysTyrThrValSerIleLeuArg354045AlaLeuAlaLeuIleThrGlyMetProIleSerAspValLeuPheGlu505560LeuGluAspLeuGluLysAsnAlaAspAspLeuAlaGlyPheLysGln65707580LeuLeuAspThrHisLysLeuSerPheProAlaHisGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerValAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluSerGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluPheMet14523149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽23MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysThrLeuAsnLysTyrThrValSerIleLeuArg354045AlaLeuAlaLeuIleThrGlyMetProIleSerAspValLeuPheGlu505560LeuGluAspLeuGluLysAsnAlaAspAspLeuAlaGlyPheLysGln65707580LeuLeuAspThrHisLysLeuSerPheProAlaHisGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerValAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluSerGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluPheIle14524149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽24MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysThrLeuAsnLysTyrThrValSerIleLeuArg354045AlaLeuAlaLeuIleThrGlyMetProIleSerAspValLeuPheGlu505560LeuGluAspLeuGluLysAsnAlaAspAspLeuAlaGlyPheLysGln65707580LeuLeuAspThrHisLysLeuSerPheProAlaHisGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerValAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluSerGluThrHisValAspIleGluLys115120125AspValGlnLysAlaLeuGluAsnAlaIleAlaValLeuLysGluLys130135140LysGluGluLeuLeu14525125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽25MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysProLeuAsnLysTyrThrValSerIleLeuArg354045SerLeuSerLeuIleSerGlyLeuSerValSerAspValLeuPheGlu505560LeuGluAspIleGluLysAsnSerAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsn11512012526149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽26MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsnIleLysLys115120125AspValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuLeu14527149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽27MetSerIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsnIleLysLys115120125AspValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuLeu14528149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽28MetSerIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsnIleGluLys115120125AspValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuLeu14529149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽29MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysThrGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAsnLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheAspSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsnIleGluLys115120125AspValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuLeu14530149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽30MetSerIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsnIleGluLys115120125AspValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14531149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽31MetAsnIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysThrGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerHisAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysHisLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsnIleGluLys115120125AspValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuLeu14532149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽32MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysThrGlyIleSerThrPheAspValLeuIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14533149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽33MetThrIleLysLeuLeuAspGluPheLeuLysLysHisSerLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValLeuIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14534149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽34MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValLeuIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14535149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽35MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValLeuIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluCysAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14536149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽36MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14537149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽37MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysThrGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysAsnTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluCysAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14538149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽38MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheAsnGlu505560LeuGluGluLeuGluLysAsnTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheSerAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14539149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽39MetThrIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluCysAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14540149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽40MetSerIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14541149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽41MetSerIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluCysAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuLeu14542149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽42MetThrIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysThrGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisThrAspIleGluLys115120125AspValLysLysThrLeuAsnAsnAlaIleAlaValLeuLysGluLys130135140LysGluGluLeuLeu14543149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽43MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluCysAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14544149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽44MetSerIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheMetArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14545149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽45MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheLysGlu505560LeuGluGluLeuGluLysAsnTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheSerAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14546149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽46MetSerIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuSerGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14547149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽47MetSerIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaSerIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14548149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽48MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysThrGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerHisAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysHisLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14549149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽49MetSerIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluCysAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14550149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽50MetSerIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluCysAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspIleArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14551149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽51MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrThrValSerPheLeuArg354045ThrLeuSerMetCysValGlyMetSerThrValAspValPheIleGlu505560LeuAlaGluLeuGluLysAsnTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluSerGluThrHisValAspIleGluLys115120125AspValLysLysAlaLeuAsnAsnAlaIleAlaValLeuLysGluLys130135140LysGluGluLeuLeu14552149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽52MetSerIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuAsnAsnAlaIleAlaValLeuLysGluLys130135140LysGluGluLeuLeu14553149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽53MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysTyr65707580LeuLeuAspLysHisLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLys130135140LysAsnGluLeuIle14554149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽54MetAsnIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysThrGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysHisLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisAlaAspIleGluLys115120125AspValLysLysAlaLeuAsnAsnAlaIleAlaValLeuLysAlaLys130135140LysGluGluLeuLeu14555130PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽55MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAspLeuThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuLys202530AspGlnAsnGluLysThrLeuAsnLysTyrThrValSerIleLeuArg354045AlaLeuAlaLeuIleThrGlyMetProIleSerAspValLeuPheGlu505560LeuGluAspLeuGluLysAsnAlaAspAspLeuAlaGlyPheLysGln65707580LeuLeuAspThrHisLysLeuSerPheProAlaHisGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerValAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluSerGluThrHisValAspIleGluLys115120125AspVal13056134PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽56ThrArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeu151015AsnAspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeu202530ArgAlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIle354045GluLeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLys505560HisLeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGlu65707580LeuTyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeu859095ProPheThrPheAsnArgPheGluAsnGluGluHisValAsnIleLys100105110LysAspValCysLysAlaLeuGluAsnAlaIleThrValLeuLysGlu115120125LysLysAsnGluLeuLeu13057149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽57MetLysIleAsnLeuLeuAspGluPheLeuLysArgHisAsnIleThr151015ArgTyrArgLeuSerLysLeuAlaGlyIleSerGlnAsnThrLeuLys202530AspTyrAsnGluLysSerLeuAsnLysTyrThrValSerPheLeuArg354045SerLeuSerPheValThrGlyGluAspValThrAspValLeuIleGlu505560LeuAlaGluLeuGluLysGlyTyrAspAspLeuAlaGlyPheLysTyr65707580LeuLeuAspLysTyrLysLeuSerPheProAlaLeuGluPheGluLeu859095TyrCysIleIleLysGluPheGluSerAlaAsnIleGluIleSerPro100105110PheThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLys115120125AspValLysLysAlaLeuGlnAsnAlaValThrValLeuGluGluArg130135140LysGluGluLeuLeu14558149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽58MetLysAsnAsnLeuLeuAspThrPheLeuLysArgHisAspIleThr151015ArgTyrArgLeuSerLysLeuAlaGlyIleSerGlnAsnThrLeuLys202530AspTyrAsnGluLysSerLeuAsnLysTyrThrValSerLeuLeuArg354045SerLeuSerPheValThrGlyGluSerIleThrAspValLeuLeuGlu505560LeuAlaGluIleGluLysAspTyrAspAspLeuAlaGlyPheLysTyr65707580LeuLeuAspLysTyrLysLeuSerPheProAlaLeuGluPheGluLeu859095TyrCysIleIleLysGluPheGluSerAlaAsnValGluIleSerPro100105110PheThrPheAsnArgPheGluAsnGluThrHisAlaAspIleGluLys115120125AspValLysLysAlaLeuAsnAsnAlaIleThrValLeuLysGluLys130135140LysGluGluLeuLeu14559149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽59MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysTyr65707580LeuLeuAspLysHisLysLeuSerPheProThrGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisAlaAspIleGluLys115120125AspValLysLysAlaLeuAsnAsnAlaIleAlaValLeuGluGluLys130135140LysGluGluLeuLeu14560149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽60MetLysIleAsnLeuLeuAspAlaPheLeuLysArgHisAsnIleThr151015ArgTyrArgLeuSerLysLeuAlaGlyIleSerGlnAsnThrLeuLys202530AspTyrAsnGluLysSerLeuAsnLysTyrThrValSerPheLeuArg354045SerLeuSerPheValThrGlyGluAspValThrAspValLeuIleGlu505560LeuAlaGluLeuGluLysGlyTyrAspAspLeuAlaGlyPheLysTyr65707580LeuLeuAspLysTyrLysLeuAlaPheProAlaLeuGluPheGluLeu859095TyrCysLeuIleLysGluPheGluAlaAlaAsnIleGluValSerPro100105110PheThrPheAsnArgPheGluAsnGluThrHisAlaAspIleGluLys115120125AspValLysLysAlaLeuLysAsnAlaIleIleValLeuLysGluLys130135140LysGluGluLeuLeu14561133PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽61MetSerIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsnIleGluLys115120125AspValCysLysAla13062149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽62MetLysIleAsnLeuLeuAspGluPheLeuLysArgHisAsnIleThr151015ArgTyrArgLeuSerLysLeuAlaGlyIleSerGlnAsnThrLeuLys202530AspTyrThrGluLysSerLeuAsnLysTyrThrValSerPheLeuArg354045SerLeuSerPheAlaThrGlyGluSerValThrAspIleLeuLeuGlu505560LeuAlaGluLeuGluLysAspTyrAspAspLeuAlaGlyPheLysTyr65707580LeuLeuAspLysTyrLysLeuAlaPheProAlaLeuGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluIleSerPro100105110PheThrPheAsnArgPheGluSerGluThrHisThrAspIleGluLys115120125AspValLysLysAlaLeuGlnAsnAlaValThrValLeuGluGluArg130135140LysGluGluLeuLeu14563149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽63MetSerIleLysLeuLeuAspGluPheLeuLysLysHisSerLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045SerLeuSerPheAlaThrGlyGluSerValThrAspIleLeuLeuGlu505560LeuAlaGluLeuGluLysAspTyrAspAspLeuAlaGlyPheLysTyr65707580LeuLeuAspLysTyrLysLeuAlaPheProAlaLeuGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluIleSerPro100105110PheThrPheAsnArgPheGluSerGluThrHisThrAspIleGluLys115120125AspValLysLysAlaLeuGlnAsnAlaValThrValLeuGluGluArg130135140LysGluGluLeuLeu14564159PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽64MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysTyr65707580LeuLeuAspLysHisLysLeuSerPheProThrGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluThrHisAlaAspIleGluLys115120125AspValLysLysAlaLeuAsnAsnAlaIleAlaValLeuGluGluLys130135140LysArgArgThrValIleLysThrIleAspTyrTyrAspTyrSer14515015565148PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽65MetAsnAsnPheAlaPheIleThrSerPheAsnTyrGlnGlnProArg151015TyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsnAsp202530TyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArgAla354045LeuSerMetCysAlaGlyIleSerThrPheAspValLeuIleGluLeu505560AlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHisLeu65707580LeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeuTyr859095CysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuProPhe100105110ThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLysAsp115120125ValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLysLys130135140AsnGluLeuIle14566149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽66MetLysThrAsnLeuLeuAspThrPheLeuLysArgHisGlyIleThr151015ArgTyrArgLeuSerLysLeuAlaGlyIleSerGlnAsnThrLeuLys202530AspTyrThrGluLysSerLeuAsnLysTyrThrValSerPheLeuArg354045SerLeuSerPheValThrGlyGluAspValThrAspValLeuLeuGlu505560LeuAlaGluIleGluAsnGlyTyrAspAspLeuAlaGlyPheLysTyr65707580LeuLeuAspLysTyrLysLeuSerPheProAlaLeuGluPheGluLeu859095TyrCysIleIleLysGluPheGluSerAlaAsnIleGluIleSerPro100105110PheThrPheAsnArgPheGluAsnGluThrHisAlaAspIleGluLys115120125AspValLysLysAlaLeuLysAsnAlaValThrValLeuGluGluArg130135140LysGluGluLeuLeu14567148PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽67MetAsnAsnPheAlaPheIleThrSerPheAsnTyrGlnGlnProArg151015TyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsnAsp202530TyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArgAla354045LeuSerMetCysAlaGlyIleSerThrPheAspValLeuIleGluLeu505560AlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHisLeu65707580LeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeuTyr859095CysLeuIleLysGluPheGluCysAlaAsnIleGluValLeuProPhe100105110ThrPheAsnArgPheGluAsnGluThrHisValAspIleGluLysAsp115120125ValArgLysAlaLeuGluAsnAlaIleThrValLeuLysGluLysLys130135140AsnGluLeuIle14568125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽68MetSerIleLysLeuLeuAspGluPheLeuLysLysHisAsnLysThr151015ArgTyrGlnLeuSerLysLeuThrGlyIleSerGlnAsnThrLeuAsn202530AspTyrAsnLysLysGluLeuAsnLysTyrSerValSerPheLeuArg354045AlaLeuSerMetCysAlaGlyIleSerThrPheAspValPheIleGlu505560LeuAlaGluLeuGluLysSerTyrAspAspLeuAlaGlyPheLysHis65707580LeuLeuAspLysTyrLysLeuSerPheProAlaGlnGluPheGluLeu859095TyrCysLeuIleLysGluPheGluSerAlaAsnIleGluValLeuPro100105110PheThrPheAsnArgPheGluAsnGluGluHisValAsn11512012569123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽69MetThrLeuThrArgAlaGlnLysLysTyrAlaGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerThrProAspPheAla202530LysGluValLeuHisAspSerAspTyrValValIleThrLysAsnGlu354045LysTyrAlaValAlaLeuCysSerLeuSerThrAspGluCysGluTyr505560AspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThrVal65707580AspValAsnGlyValThrTyrTyrIleAsnIleValGluThrAsnAsp859095IleAspAspLeuGluIleAlaThrAspGluAspGluMetLysSerGly100105110AsnGlnGluIleIleLeuLysSerGluLeuLys11512070123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽70MetThrLeuThrArgAlaGlnLysLysTyrAlaGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerThrProAspPheAla202530LysGluValLeuHisAspSerAspTyrValValIleThrLysAsnGlu354045LysTyrAlaValAlaLeuCysSerLeuSerThrAspGluCysGluTyr505560AspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThrVal65707580AspValAsnGlyValThrTyrTyrIleAsnIleValGluThrAsnAsp859095IleAspAspLeuGluIleAlaThrAspGluAspGluMetLysSerGly100105110AsnGlnGluIleIleLeuLysSerGluLeuAsn11512071123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽71MetThrIleThrArgAlaGlnLysLysTyrAlaGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerThrProAspPheAla202530LysGluValLeuHisAspSerAspTyrValValIleThrLysAsnGlu354045LysTyrAlaValAlaLeuCysSerLeuSerThrAspGluCysGluTyr505560AspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThrVal65707580AspValAsnGlyValThrTyrTyrIleAsnIleValGluThrAsnAsp859095IleAspAspLeuGluIleAlaThrAspGluAspGluMetLysSerGly100105110AsnGlnGluIleIleLeuLysSerGluLeuLys11512072124PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽72LysMetThrLeuThrArgAlaGlnLysLysTyrAlaGluAlaMetHis151015GluPheIleAsnMetValAspAspPheGluGluSerThrProAspPhe202530AlaLysGluValLeuHisAspSerAspTyrValValIleThrLysAsn354045GluLysTyrAlaValAlaLeuCysSerLeuSerThrAspGluCysGlu505560TyrAspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThr65707580ValAspValAsnGlyValThrTyrTyrIleAsnIleValGluThrAsn859095AspIleAspAspLeuGluIleAlaThrAspGluAspGluMetLysSer100105110GlyAsnGlnGluIleIleLeuLysSerGluLeuAsn11512073122PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽73MetThrLeuThrArgAlaGlnLysLysTyrAlaGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerThrProAspPheAla202530LysGluValLeuHisAspSerAspTyrValValIleThrLysAsnGlu354045LysTyrAlaValAlaLeuCysSerLeuSerThrAspGluCysGluTyr505560AspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThrVal65707580AspValAsnGlyValThrTyrTyrIleAsnIleValGluThrAsnAsp859095IleAspAspLeuGluIleAlaThrAspGluAspGluMetLysSerGly100105110AsnGlnGluIleIleLeuLysSerGluLeu11512074123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽74MetThrLeuThrArgAlaGlnLysLysTyrAlaGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerThrProAspPheAla202530LysGluValLeuHisAspSerAspTyrValValIleThrLysAsnGlu354045LysTyrAlaValAlaLeuCysSerLeuSerThrAspGluCysGluTyr505560AspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThrVal65707580AspValAsnGlyValThrTyrTyrIleAsnIleValGluThrAsnAsp859095IleAspAspLeuGluIleAlaThrAspGluAspGluMetLysSerAsp100105110AsnGlnGluIleIleLeuLysSerGluLeuLys11512075123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽75MetThrIleThrArgAlaGlnLysLysTyrAlaGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerThrProAspPheAla202530LysGluValLeuHisAspSerAspTyrValValIleThrLysAsnGlu354045LysTyrAlaValAlaLeuCysSerLeuSerThrAspGlyCysGluTyr505560AspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThrVal65707580AspValAsnGlyValThrTyrTyrIleAsnIleValGluThrAsnAsp859095IleAspAspLeuGluIleAlaThrAspGluAspGluMetLysSerGly100105110AsnGlnGluIleIleLeuLysSerGluLeuLys11512076123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽76MetThrLeuThrArgAlaGlnLysLysTyrAlaGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerThrProAspPheAla202530LysGluValLeuHisAspSerAspTyrValValIleThrLysAsnGlu354045LysTyrAlaValAlaLeuCysSerLeuSerThrAspGluCysGluTyr505560AspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThrVal65707580AspValAsnGlyValThrTyrTyrIleAsnIleValGluThrLysAsp859095IleAspAspLeuGluIleAlaThrAspGluAspGluMetLysSerAsp100105110AsnGlnGluIleIleLeuLysSerGluLeuLys11512077123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽77MetThrIleThrThrAlaGlnLysLysTyrAlaGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerThrProAspPheAla202530LysGluValLeuHisAspSerAspTyrValValIleThrLysAsnGlu354045LysTyrAlaValAlaLeuCysSerLeuSerThrAspGluCysGluTyr505560AspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThrVal65707580AspValAsnGlyValThrTyrTyrIleAsnIleValGluThrAsnAsp859095IleAspAspLeuGluIleAlaThrAspGluAspGluMetLysSerGly100105110AsnGlnGluIleIleLeuLysSerGluLeuLys11512078123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽78MetThrIleThrThrAlaGlnArgLysTyrAsnGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerThrProAspPheAla202530LysGluValLeuHisAspSerAspTyrValValIleThrLysAsnGlu354045LysTyrAlaValAlaLeuCysSerLeuSerThrAspGluCysGluTyr505560AspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThrVal65707580AspValAsnGlyValThrTyrTyrIleAsnIleValGluThrAsnAsp859095IleAspAspLeuGluIleAlaThrAspGluAspGluMetLysSerGly100105110AsnGlnGluIleIleLeuLysSerGluLeuLys11512079123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽79MetThrIleThrThrAlaGlnArgLysTyrAsnGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerThrProAspPheAla202530LysGluValLeuHisAspCysAspTyrValValValThrLysAsnGlu354045LysTyrAlaValAlaLeuCysThrLeuSerThrAspGluCysGluTyr505560AspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThrVal65707580AsnValAsnGlyValThrTyrTyrIleAsnIleValGluThrAsnAsp859095IleAspAspLeuGluIleAlaThrAspGluAspGluMetLysSerAsp100105110AsnGlnGluIleIleLeuLysSerGluLeuLys11512080123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽80MetThrIleThrThrAlaGlnArgLysTyrAsnGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerThrProAspPheAla202530LysGluValLeuHisAspCysAspTyrValValValThrLysAsnGlu354045LysTyrAlaValAlaLeuCysThrLeuSerThrAspGluCysGluTyr505560AspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThrVal65707580AsnValAsnGlyValThrTyrTyrIleAsnIleValGluThrAsnAsp859095IleAspAspLeuGluIleAlaThrAspGluAspGluMetLysSerAsp100105110AsnGlnLysIleIleLeuLysSerGluLeuLys11512081123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽81MetThrLeuThrArgAlaGlnLysLysTyrAlaGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerThrProAspPheAla202530LysGluValLeuHisAspSerAspTyrValValIleThrLysAsnGlu354045LysTyrAlaValAlaLeuCysSerLeuSerThrAspGluCysGluTyr505560AspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThrVal65707580AspValAsnGlyValThrTyrTyrIleAsnIleValGluThrLysAsp859095IleAspAspLeuGluIleAlaThrAspGluAspLysGluLysHisAsp100105110LysGlnGluValIleIleLysSerGluLeuAsn11512082110PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽82MetHisGluPheIleAsnMetValAspAspPheGluGluSerThrPro151015AspPheAlaLysGluValLeuHisAspSerAspTyrValValIleThr202530LysAsnGluLysTyrAlaValAlaLeuCysSerLeuSerThrAspGlu354045CysGluTyrAspThrAsnLeuTyrLeuAspGluLysLeuValAspTyr505560SerThrValAspValAsnGlyValThrTyrTyrIleAsnIleValGlu65707580ThrAsnAspIleAspAspLeuGluIleAlaThrAspGluAspGluMet859095LysSerGlyAsnGlnGluIleIleLeuLysSerGluLeuLys10010511083122PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽83MetThrLeuThrArgAlaGlnLysLysTyrAlaGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerLysProAsnPheAla202530LysGluValLeuHisAspSerAspTyrValValIleThrLysAsnGlu354045LysTyrAlaValAlaLeuCysSerLeuSerThrAspGluCysGluTyr505560AspThrAsnLeuTyrLeuAspGluLysLeuValAspTyrSerThrVal65707580AspValAsnGlyValThrTyrTyrIleAsnIleValValThrAsnGlu859095AspAspPheLysLeuAlaThrAspLysAspLysGluLysHisAspLys100105110GlnGluValIleValLysSerGluLeuAsn11512084122PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽84MetThrLeuThrThrAlaGlnArgLysTyrAsnGluAlaMetHisGlu151015PheIleAsnMetValAspAspPheGluGluSerThrProGluPheSer202530LysGluValLeuAsnAspSerAspTyrValValIleThrLysAsnGlu354045LysTyrAlaGlyAlaLeuCysHisValSerThrAspGluCysGluAsp505560GlySerAsnLeuTyrIleAspGluLysLeuIleAspTyrSerThrLeu65707580AsnValGlyGlyValThrTyrTyrIleAsnIleValGluArgCysGlu859095AspAspLeuGluIleAlaThrAspGluAspLysMetLysSerAspAsn100105110GlnGluIleIleLeuLysAsnGluLeuAsn11512085103PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽85MetValAspAspPheGluGluSerThrProGluPheSerLysGluVal151015LeuAsnAspSerAspTyrValValIleThrLysAsnGluLysTyrAla202530GlyAlaLeuCysHisValSerThrAspGluCysGluAspGlySerAsn354045LeuTyrIleAspGluLysLeuIleAspTyrSerThrLeuAsnValGly505560GlyValThrTyrTyrIleAsnIleValGluArgCysGluAspAspLeu65707580GluIleAlaThrAspGluAspLysMetLysSerAspAsnGlnGluIle859095IleLeuLysAsnGluLeuAsn10086116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽86MetThrLeuThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleThrSerLysLysLeuGluGlnThrProGluPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuValIleThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysMetLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValGlnSerThrCysLeuAspValGluGlyGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleValLys100105110SerGluLeuAsn11587116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽87MetThrLeuThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleThrSerLysGluLeuGluGlnThrProGluPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuValIleThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysMetLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValGlnSerThrCysLeuAspValGluGlyGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleValLys100105110SerGluLeuAsn11588116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽88MetThrLeuThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleThrSerLysGluLeuGluGlnThrProGluPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuValIleThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysMetLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValGlnSerThrCysLeuAspValGluGlyGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspLysAspLysGluLysHisAspLysGlnGluValIleValLys100105110SerGluLeuAsn11589116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽89MetThrIleThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleIleSerLysGluLeuGluGlnThrProAlaPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuValIleThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysMetLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValGlnSerThrArgLeuAspIleGluAspGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleIleLys100105110SerGluLeuAsn11590116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽90MetThrIleThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleThrSerLysGluLeuGluGlnThrProGluPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuValIleThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysMetLeuAspAspAspLysLeuTyrIle505560AspGluThrLeuValGlnSerThrCysLeuAspValGluGlyGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspLysAspLysGluLysHisAspLysGlnGluValIleIleLys100105110SerGluLeuAsn11591116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽91MetThrLeuThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleIleSerLysGluLeuGluGlnThrArgAlaPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuValIleThrLysAsnGlu354045AlaTyrAlaValAspLeuCysMetLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValGlnSerThrArgLeuAspIleGluAspGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspArgGlnGluValIleIleLys100105110SerGluLeuAsn11592116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽92MetThrIleThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleThrSerLysGluLeuGluGlnThrProGluPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuValValThrLysAsnGlu354045AlaTyrAlaAlaAlaLeuCysMetLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValGlnSerThrCysLeuAspValGluGlyGluIle65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspLysAspLysGluLysHisAspLysGlnGluValIleValLys100105110SerGluLeuAsn11593116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽93MetThrIleThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleThrSerLysGlyLeuGluGlnThrProGluPheSer202530GlnAspLeuLeuAsnAspPheAspTyrLeuValIleThrLysAsnGlu354045AlaTyrAlaAlaAlaLeuCysMetLeuAspAspGluLysLeuTyrLeu505560AspGluThrLeuValHisSerThrArgLeuAspIleGluAspAspThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleIleLys100105110ArgGluLeuAsn11594116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽94MetThrIleThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleIleSerLysGluLeuGluGlnThrProAlaPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuValIleThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysLeuLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValHisSerThrArgLeuAspIleGluAspGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleIleLys100105110SerGluLeuAsn11595116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽95MetThrIleThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleIleSerLysGluLeuGluGlnThrProAlaPheSer202530GlnAsnLeuLeuAsnAspSerAspTyrLeuValIleThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysLeuLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValHisSerThrArgLeuAspIleGluAspGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleIleLys100105110SerGluLeuAsn11596116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽96MetThrIleThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleThrSerLysGluLeuGluGlnThrProAlaPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuValIleThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysLeuLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValHisSerThrArgLeuAspIleGluAspGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleValLys100105110SerGluLeuAsn11597116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽97MetThrIleThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleIleSerLysGluLeuGluGlnThrProAlaPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuValIleThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysLeuLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValHisSerThrArgLeuAsnIleGluAspGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleIleLys100105110SerGluPheAsn11598116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽98MetThrLeuThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleThrSerLysGluLeuGluGlnThrProGluPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuAlaValThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysLeuLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValHisSerThrArgLeuAspIleGluAspGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleIleLys100105110SerGlyLeuAsn11599116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽99MetThrValThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleThrSerLysGluLeuGluGlnThrProGluPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuAlaValThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysLeuLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValHisSerThrArgLeuAspIleGluAspGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleIleLys100105110SerGluLeuAsn115100116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽100MetThrLeuThrThrValGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleThrSerLysGluLeuGluGlnThrProGluPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuAlaValThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysLeuLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValHisSerThrArgPheAspIleGluAspGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleIleLys100105110SerGluLeuAsn115101116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽101MetThrLeuThrThrValGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleThrSerLysGluLeuGluGlnThrProGluPheSer202530GlnAspSerLeuAsnAspSerAspTyrLeuAlaValThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysLeuLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValHisSerThrArgPheAspIleGluAspGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleIleLys100105110SerGluLeuAsn115102116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽102MetThrIleThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleIleSerLysGluLeuGluGlnThrProAlaPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuAlaValThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysLeuLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValHisSerThrArgLeuAspIleGluAspGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleIleLys100105110SerGlyLeuAsn115103116PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽103MetThrIleThrThrAlaGlnLysArgTyrTyrAspAlaMetAsnGlu151015PheGluAlaIleThrSerLysGluLeuGluGlnThrProAlaPheSer202530GlnAspLeuLeuAsnAspSerAspTyrLeuAlaValThrLysAsnGlu354045AlaTyrAlaValAlaLeuCysLeuLeuAspAspAspLysLeuTyrLeu505560AspGluThrLeuValHisSerThrArgLeuAspIleGluAspGluThr65707580TyrTyrIleAsnPheValValThrAsnGluAspAspPheLysLeuAla859095ThrAspGluAspLysGluLysHisAspLysGlnGluValIleIleLys100105110SerGlyLeuAsn115104130PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽104MetAlaIleThrLeuSerGlnArgLysPheTyrGluAlaIleAsnGlu151015PheGluGluMetThrGluAsnGluValValThrSerProArgIlePro202530GlnAspTyrLeuAsnAspGlyAspTyrValValIleThrLysSerGlu354045AsnTyrAlaLeuAsnLeuCysThrThrAsnLeuGluGlyPheGluAsp505560ArgHisPheLeuAspGluLysLeuIleTyrSerThrPheValGluThr65707580TyrSerGlyGluThrTyrTyrIleTyrIleThrGlnThrAlaGluPhe859095AspGluAspAspAlaValGluPheLeuAlaThrGlnGluGlnIleTyr100105110GluTyrHisLysGlnGluGluGlnLysThrValIleLeuLysMetGlu115120125LeuSer130105130PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽105MetAlaGlnThrGluAlaGlnLysIlePheTyrGluAlaIleAsnGlu151015PheGluGluMetThrAsnGluGluValValThrSerProArgIlePro202530GlnAspTyrLeuAsnAspGlyAspTyrValValIleThrLysSerGlu354045AsnTyrAlaLeuAsnLeuCysThrThrAspLeuGluGlyPheGluAsp505560ArgTyrPheLeuAspGluLysLeuIleTyrSerThrSerValGluThr65707580TyrThrAspGluThrTyrTyrIleTyrIleThrGlnThrThrGluPhe859095GluGluAspAsnAlaValGluPheLeuAlaThrGlnGluGlnIleTyr100105110GluTyrHisLysGlnGluGluGlnLysThrValIleLeuLysMetGlu115120125LeuSer130106130PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽106MetThrThrAlaArgLysLysPheTyrGlnAlaIleSerGluPheGlu151015AlaMetThrGlyLysAspValGluArgThrProGlnIleAlaAspGlu202530ValLeuAsnAspAlaGluTyrIleAlaPheThrLysThrGluLysTyr354045AlaLeuTyrLeuCysThrSerAsnValGluGlyLeuGluAspArgTyr505560PheLeuAspGluGluCysLeuAspSerThrPheLeuGluThrGluAsp65707580AsnGluThrTyrTyrIleHisPheLeuGlnGluThrGluPheSerGlu859095AspAspAsnGluAspGluLeuProLeuAlaThrGluGluGlnIleGlu100105110AlaTyrAspLysGlnGluGluLeuLysAlaValIleLeuLysLysGlu115120125LeuAsn130107129PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽107MetArgThrThrAlaGlnGluArgLeuAspAsnAlaIleAsnGluPhe151015GluGluIleThrAsnGluGluValValThrSerProArgIleProGln202530AspTyrLeuAsnAspGlyAspTyrValValIleThrLysSerGluAsn354045TyrAlaLeuAsnLeuCysThrThrAsnLeuGluGlyPheGluAspArg505560HisPheLeuAspGluLysLeuIleTyrSerThrPheValGluThrTyr65707580SerGlyGluThrTyrTyrIleTyrIleThrGlnThrAlaGluPheAsp859095GluAspAspAlaValGluPheLeuAlaThrGlnGluGlnIleTyrGlu100105110TyrHisLysGlnGluGluGlnLysThrValIleLeuLysMetGluLeu115120125Ser108129PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽108MetArgThrThrAlaGlnGluArgLeuAspAsnAlaIleAsnGluPhe151015GluGluIleThrAsnGluGluValValThrSerProArgIleProGln202530AspTyrLeuAsnAspGlyAspTyrValValIleThrLysSerGluAsn354045TyrAlaLeuAsnLeuCysThrThrAsnLeuGluGlyPheGluAspArg505560HisPheLeuAspGluLysLeuIleTyrSerThrPheValGluThrTyr65707580AlaGlyGluThrTyrTyrIleTyrIleThrGlnThrAlaGluPheAsp859095GluAspAspAlaValGluPheLeuAlaThrGlnGluGlnIleTyrGlu100105110TyrHisLysGlnGluGluGlnLysThrValIleLeuLysMetGluLeu115120125Ser109129PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽109MetArgThrThrAlaGlnGluArgLeuAspAsnAlaIleAsnGluPhe151015GluGluIleThrAsnGluGluValValThrSerProLeuIleProGln202530AspTyrLeuAsnAspGlyAspTyrValValIleThrLysSerGluAsn354045TyrAlaLeuAsnLeuCysThrThrAsnLeuGluGlyPheGluAspArg505560HisPheLeuAspGluLysLeuIleTyrSerThrPheValGluThrTyr65707580SerGlyGluThrTyrTyrIleTyrIleThrGlnThrAlaGluPheAsp859095GluAspAspAlaValGluPheLeuAlaThrGlnGluGlnIleTyrGlu100105110TyrHisLysGlnGluGluGlnLysThrValIleLeuLysMetGluLeu115120125Ser110125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽110MetPheAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheAsn151015AspSerAsnIleTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysSerPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValGluGluSerLysLysGluSerLysHisIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125111125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽111MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheAsn151015AspSerAsnIleTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysSerPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValGluGluSerLysLysGluSerLysHisIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125112125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽112MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheAsn151015AspSerAsnIleTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysSerPheSerValCysLeuLysGlyAlaTrpSerLysAla354045LysGluGluValGluGluSerLysLysGluSerLysHisIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125113125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽113MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheAsn151015AspSerAsnIleTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysSerPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValGluGluSerLysLysGluSerLysHisIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerLeuTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125114125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽114MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheAsn151015AspSerAsnValTrpLeuSerAspIleGluTrpIleSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValGluGluSerLysLysGluSerLysHisIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125115125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽115MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheAsn151015AspSerAsnIleTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysSerPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValGluGluSerLysLysGluSerLysTyrIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580ArgPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125116125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽116MetPheAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheThr151015AspSerAsnValTrpLeuSerAspIleGluTrpAlaSerTyrThrAsp202530LysGluLysSerPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValGluGluSerLysLysGluSerLysHisIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125117125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽117MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheAsn151015AspSerAsnIleTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysSerPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValGluGluPheLysLysGluSerLysTyrIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580ArgPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125118118PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽118MetLysGlnAlaTrpAsnTrpPheAsnAspSerAsnIleTrpLeuSer151015AspIleGluTrpValSerTyrThrAspLysGluLysSerPheSerVal202530CysLeuLysAlaAlaTrpSerLysAlaLysGluGluValGluGluSer354045LysLysGluSerLysTyrIleAlaLysSerGluGluLeuLysAlaTrp505560AsnTrpAlaGluArgLysLeuGlyLeuArgPheAsnIleSerAspAsp65707580GluLysPheThrSerValLysAspGluThrLysIleAsnPheGlyLeu859095SerValTrpAlaCysAlaMetLysAlaValLysLeuHisAsnAspLeu100105110PheProGlnThrAlaAla115119125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽119MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheAsn151015AsnSerAsnValTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValGluGluSerLysGluGluSerLysHisIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125120125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽120MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheAsn151015AsnSerAsnValTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuArgAlaAlaTrpSerLysAla354045LysGluGluValGluGluSerLysGluGluSerLysHisIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125121125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽121MetPheAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheThr151015AspSerAsnValTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValLysGluValGluLysGluIleLysHisIleSerLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125122125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽122MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheAsn151015AspSerAsnValTrpLeuSerAspIleGluTrpAlaSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValLysGluValGluLysGluIleLysHisIleSerLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125123125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽123MetPheAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheThr151015AspSerAsnValTrpLeuSerAspIleGluTrpAlaSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValLysGluValGluLysGluIleLysHisIleSerLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125124125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽124MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheThr151015AspSerAsnValTrpLeuSerAspIleGluTrpAlaSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValLysGluValGluLysGluIleLysHisIleSerLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125125125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽125MetPheAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheThr151015AspSerAsnValTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluPheLysGluValGluLysGluIleLysHisIleSerLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAsnGlu859095ThrLysMetAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125126125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽126MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheThr151015AspSerAsnValTrpLeuSerAspIleGluTrpAlaSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValLysGluValGluLysGluIleLysHisIleSerLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrValAla115120125127125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽127MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheAsn151015AspSerAsnValTrpLeuSerAspIleGluTrpAlaSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValLysGluValGluLysGluIleLysHisIleSerLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisSerHisLeuPheProGlnThrAlaAla115120125128125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽128MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheThr151015AspSerAsnValTrpLeuSerAspIleGluTrpAlaSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValLysGluValGluLysGluIleLysHisIleSerLys505560ArgGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrValAla115120125129125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽129MetPheAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheThr151015AspSerAsnValTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValLysGluValGluLysGluIleLysHisIleSerLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580ArgPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysGlnHisPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125130125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽130MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheAsn151015AspSerAsnIleTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysSerPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValGluGluSerLysLysGluSerLysHisIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluIleLysLeuGlyLeu65707580ArgPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysMetAsnPheAspLeuAsnValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125131125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽131MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheThr151015AspSerAsnValTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValLysGluValGluLysGluIleLysHisIleSerLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580ArgPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysGlnHisPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125132125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽132MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnCysPheAsn151015AspSerAsnValTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluIleGluLysSerLysLysGluSerLysHisIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580ArgPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysGlnHisPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125133125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽133MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnCysPheAsn151015AspSerAsnValTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluIleGluGluSerLysLysGluSerLysHisIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580ArgPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysGlnHisPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125134125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽134MetTyrAsnLysSerGluIleMetGlnGlnAlaTrpAsnTrpPheArg151015AspSerSerValTrpLeuSerAspIleGluTrpValSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValGluGluSerLysLysGluSerLysHisIleAlaLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluSerLysLeuGlyLeu65707580ArgPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrAlaAla115120125135125PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽135MetTyrAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheThr151015AspSerAsnValTrpLeuSerAspIleGluTrpAlaSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValLysGluValGluLysGluIleLysHisIleSerLys505560ArgGluGluLeuLysAlaTrpAsnLeuAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsnAspLeuPheProGlnThrValAla115120125136117PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽136MetPheAsnLysAlaGluIleMetLysGlnAlaTrpAsnTrpPheThr151015AspSerAsnValTrpLeuSerAspIleGluTrpAlaSerTyrThrAsp202530LysGluLysThrPheSerValCysLeuLysAlaAlaTrpSerLysAla354045LysGluGluValLysGluValGluLysGluIleLysHisIleSerLys505560SerGluGluLeuLysAlaTrpAsnTrpAlaGluArgLysLeuGlyLeu65707580HisPheAsnIleSerAspAspGluLysPheThrSerValLysAspGlu859095ThrLysIleAsnPheGlyLeuSerValTrpAlaCysAlaMetLysAla100105110ValLysLeuHisAsn115137128PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽137MetLysLysValThrTyrAspLysSerGlyIleMetLysGluAlaTrp151015AsnLeuPheAsnAsnAspAspIleThrLeuAlaAspPheGluHisLeu202530GlyTrpMetGluTrpLysSerGluLysThrPheAlaLeuCysLeuLys354045GluAlaTrpGlyArgGluLysGluValValGluArgValAsnGlnLys505560PheAlaAsnAlaGluThrSerGluGluAlaLysAlaTrpAspTrpAla65707580CysLysLysLeuGlyValAlaPheGluMetAspAlaTyrThrLysMet859095ThrAsnValGluAspMetGluLysGluAlaTrpProGlyThrSerVal100105110TrpSerLeuAlaMetArgAlaValLysLeuHisMetGluValAlaAla115120125138123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽138MetArgTyrAsnLysSerGluIleMetLysAsnAlaTrpAlaMetPhe151015AsnSerCysAsnTrpGlyAlaGluAsnPheLysPheValSerValGlu202530GluLysThrPheAlaAlaCysLeuLysGluAlaTrpAlaGluGluLys354045GluTyrValGluGluLysIleLysGluSerAlaAsnAlaProLysSer505560GluGluAlaLysAlaTrpAspTrpAlaCysArgLysLeuAsnAlaAsn65707580LysLeuGlnAsnValGluAlaThrAspLysValAlaTrpValSerGlu859095MetAlaLysGluMetTrpSerSerAsnIleTrpAlaGlnAlaIleLys100105110AlaValLysLeuHisIleLysLeuPheAlaAla115120139126PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽139MetLysTyrAsnLysSerGluIleMetLysAsnAlaTrpThrMetPhe151015AsnAspAspAsnPheAspThrSerTyrTyrGluTyrAlaThrAlaGlu202530ValTyrGlyGlnLysThrPheSerGluCysLeuLysGluSerTrpGly354045ArgGluLysAlaTyrGlnGluGluLysGluLysArgLeuValAspAla505560ProLysSerGluGluAlaLysAlaTrpAspTrpAlaCysArgLysLeu65707580AsnValAsnGluLeuGlnAsnIleAspAlaThrAspLysValPheTyr859095ValGluGlyMetAlaLysGluMetTrpSerSerAsnValTrpAlaGln100105110AlaIleLysAlaValLysLeuHisIleGluLeuPheValAla115120125140129PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽140MetLysLysValAlaTyrAspLysSerGlyIleMetLysGluAlaTrp151015GluMetPheAsnArgAsnTyrGlnIleCysAspPheGluTyrAlaAsp202530PheSerGlyArgGluTyrPheGluTyrAlaSerPheAlaAspCysLeu354045LysGluAlaTrpAlaHisGluLysGluValValGluArgValAsnGln505560LysTyrAlaAspAlaGluThrSerGluGluValLysAlaTrpAspTrp65707580AlaCysLysLysLeuGlyValAlaPheGluMetAspAlaTyrThrLys859095IleThrAsnValGluGlyMetGluLysGluAlaTrpProGlyThrSer100105110ValTrpSerLeuAlaMetArgAlaValLysLeuHisMetGluValAla115120125Ala141123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽141MetAlaLysTyrAsnLysSerGluIleMetThrGlnAlaTrpThrLeu151015PheAsnSerAspAsnPheAspThrCysAspTyrGluTyrThrThrAla202530LeuValTyrGlyGlnLysAsnPheSerAspCysLeuLysGluAlaTrp354045GlyArgGluLysAlaIleValGluArgMetAlaGluGlnGluAlaAsn505560AlaProLeuSerGluGluAlaLysAlaTrpAspTrpAlaCysArgLys65707580LeuGlyValThrAlaGluValThrAlaValGluLysValArgTyrVal859095AspAspMetAlaLysGluMetTrpSerAlaAsnValTrpLysGlnAla100105110IleLysAlaValGlnLeuTyrAlaThrValAla115120142123PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽142MetAlaLysTyrAsnLysSerGluIleMetThrGlnAlaTrpThrLeu151015PheAsnSerAspAsnPheAspThrCysAspTyrGluTyrAlaThrAla202530LeuValTyrGlyGlnLysThrPheSerAspCysLeuLysGluAlaTrp354045GlyArgGluLysAlaIleValGluArgMetAlaGluLysGluAlaAsn505560AlaProLeuSerGluGluAlaLysAlaTrpAspTrpAlaCysArgLys65707580LeuGlyValThrAlaGluValThrAlaValGluLysValArgTyrVal859095AspAspMetAlaLysGluMetTrpSerThrAsnValTrpLysGlnAla100105110IleLysAlaValGlnLeuTyrAlaThrValAla115120143124PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽143MetAlaLysTyrAsnLysSerGluIleMetLysAsnAlaTrpAlaMet151015PheAsnSerTyrGluTrpAspValGluAsnPheLysPheValSerAla202530GluAsnLysThrPheSerAsnCysLeuLysGluAlaTrpAlaGluGlu354045LysGluTyrValGluArgLysAlaLysGluThrAlaGluAlaProLys505560SerGluGluAlaLysAlaTrpAspTrpAlaCysArgLysLeuAsnVal65707580AsnAspLeuGlnAsnIleAspAlaThrAspLysValPheTyrValVal859095AspMetGlnLysGluMetTrpThrSerAsnValTrpAlaGlnAlaIle100105110LysAlaValGluLeuTyrValLysLeuGlyLeuAla115120144124PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽144MetThrLysTyrAsnLysSerGluIleMetLysAsnAlaTrpAlaMet151015PheAsnSerTyrGluTrpAspValGluAsnPheLysPheValSerAla202530GluAsnLysThrPheSerAsnCysLeuLysGluAlaTrpAlaGluGlu354045LysGluTyrValGluArgLysAlaLysGluThrAlaGluAlaProArg505560SerGluGluAlaLysAlaTrpAspTrpAlaCysArgLysLeuAsnVal65707580AsnAspLeuGlnAsnIleAspAlaThrAspLysValPheTyrValVal859095AspMetGlnLysGluMetTrpThrSerAsnValTrpAlaGlnAlaIle100105110LysAlaValGluLeuTyrValLysLeuGlyLeuAla115120145124PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽145MetThrLysTyrAsnLysSerGluIleMetLysAsnAlaTrpAlaMet151015PheAsnSerTyrGluTrpAspValGluAsnPheLysPheValSerAla202530GluAsnLysThrPheSerAsnCysLeuLysGluAlaTrpAlaGluGlu354045LysGluTyrValGluArgLysAlaLysGluThrAlaGluAlaProLys505560SerGluGluAlaLysAlaTrpAspTrpAlaCysArgLysLeuAsnVal65707580AsnAspLeuGlnAsnIleAspAlaThrAspLysValPheTyrValVal859095AspMetGlnLysGluMetTrpThrSerAsnIleTrpAlaGlnAlaIle100105110LysAlaValGluLeuTyrValLysLeuGlyLeuAla115120146124PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽146MetThrLysTyrAsnLysSerGluIleMetLysAsnAlaTrpAlaMet151015PheAsnSerTyrGluTrpAspValGluAsnPheLysPheValSerAla202530GluAsnLysThrPheSerAsnCysLeuLysGluAlaTrpAlaGluGlu354045LysGluTyrValGluArgLysAlaLysGluAlaAlaGluAlaSerLys505560SerGluGluAlaLysAlaTrpAspTrpAlaCysArgLysLeuAsnVal65707580AsnAspLeuGlnAsnIleAspAlaThrAsnLysValPheTyrValVal859095AspMetGlnLysGluMetTrpThrSerAsnValTrpAlaGlnAlaIle100105110LysAlaValGluLeuTyrValLysLeuGlyLeuAla11512014787PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽147MetAsnIleAsnAspLeuIleArgGluIleLysAsnLysAspTyrThr151015ValLysLeuSerGlyThrAspSerAsnSerIleThrGlnLeuIleIle202530ArgValAsnAsnAspGlyAsnGluTyrValIleSerGluSerGluAsn354045GluSerIleValGluLysPheIleSerAlaPheLysAsnGlyTrpAsn505560GlnGluTyrGluAspGluGluGluPheTyrAsnAspMetGlnThrIle65707580ThrLeuLysSerGluLeuAsn8514887PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽148MetAsnIleAsnGluLeuIleArgGluIleLysAsnLysAspTyrThr151015AlaLysLeuSerGlyThrAspSerAsnSerIleThrGlnLeuIleIle202530ArgValAsnAsnAspGlyAsnGluTyrValIleSerGluSerGluAsn354045GluSerIleValGluLysPheIleSerAlaPheLysAsnGlyTrpAsn505560GlnGluTyrGluAspGluGluGluPheTyrAsnAspMetGlnThrIle65707580ThrLeuLysSerGluLeuAsn8514987PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽149MetAsnIleAsnGluLeuIleArgGluValLysAsnLysAspTyrThr151015AlaLysLeuSerGlyThrAspSerAsnSerIleThrGlnLeuIleIle202530ArgValAsnAsnAspGlyAsnGluTyrValIleSerGluSerGluAsn354045GluSerIleValGluLysPheIleSerAlaPheLysAsnGlyTrpAsn505560GlnGluTyrGluAspGluGluGluPheTyrAsnAspMetGlnThrIle65707580ThrLeuLysSerGluLeuAsn8515087PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽150MetAsnIleAsnGluLeuIleArgGluIleLysAsnLysAspTyrThr151015AlaLysLeuSerGlyThrAspSerAsnSerIleAlaGlnLeuIleIle202530ArgValAsnAsnAspGlyAsnGluTyrValIleSerGluSerGluAsn354045GluSerIleValGluLysPheIleSerAlaPheLysAsnGlyTrpAsn505560GlnGluTyrGluAspGluGluGluPheTyrAsnAspMetGlnThrIle65707580ThrLeuLysSerGluLeuAsn8515187PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽151MetAsnIleAsnGluLeuIleArgGluIleLysAsnLysAspTyrThr151015AlaLysLeuSerGlyThrAspSerAsnSerIleThrGlnLeuIleIle202530HisValAsnAsnAspGlyAsnGluTyrValIleSerGluSerGluAsn354045GluSerIleValGluLysPheIleSerAlaPheLysAsnGlyTrpAsn505560GlnGluTyrGluAspGluGluGluPheTyrAsnAspMetGlnThrIle65707580ThrLeuLysSerGluLeuAsn8515287PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽152MetAsnIleAsnAspLeuIleArgGluIleLysAsnLysAspTyrThr151015ValLysLeuSerGlyThrAspSerAsnSerIleThrGlnLeuIleIle202530AsnValAsnAsnAspGlyAsnGluTyrGlyIleSerGluSerAsnPhe354045GluSerIleValGluLysPheValSerAsnPheGluAsnGlyTrpAsp505560GlyAlaTyrGluAspGluGluGluPheTyrAsnAspMetGlnAlaIle65707580IleLeuLysSerGluLeuAsn8515387PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽153MetAsnIleAsnAspLeuIleArgGluIleLysAsnLysAspTyrThr151015ValLysLeuSerGlyThrAspSerAsnSerIleThrGlnLeuIleIle202530AsnValAsnAsnAspGlyAsnGluTyrGlyIleSerGluSerAsnPhe354045GluSerIleValGluLysPheValSerAsnPheGluAsnGlyTrpAsp505560GlyAlaTyrGluAspGluGluGluPheTyrAsnAspMetGlnAlaIle65707580IleLeuLysSerGluSerAsn8515487PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽154MetAsnIleSerGluLeuIleArgGluIleLysAsnLysAspTyrThr151015ValArgLeuGluGlyThrAspAspAsnSerIleThrLysLeuIleIle202530AspValAspAsnAspGlyAsnGluTyrValIleSerGluSerLysAsn354045GluSerIleAlaGluLysPheAlaSerThrPheLysAsnGlyTrpAsn505560LysGluTyrGluAspGluGluGluPheTyrAsnAspMetGlnSerIle65707580IleLeuLysSerGluLeuAsn8515587PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽155MetAsnIleSerGluLeuIleArgGluIleLysAsnLysAspTyrAla151015ValArgLeuGluGlyThrAspAspAsnSerIleThrLysLeuIleIle202530AspValAspAsnAspGlyAsnGluTyrValIleSerGluSerLysAsn354045GluSerIleAlaGluLysPheAlaSerThrPheLysAsnGlyTrpAsn505560LysGluTyrGluAspGluGluGluPheTyrAsnAspMetGlnSerIle65707580IleLeuLysSerGluLeuAsn85156160PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽156MetAlaGlyTyrLeuLysArgTyrAlaGluAspArgGlyTrpThrLeu151015TyrArgLeuAlaLysGluSerHisLeuSerAspSerThrLeuArgThr202530AlaAspLeuThrThrLeuAsnLysLeuSerValIleAsnIleLysLys354045IleSerGluAlaValGlyGluThrProGlyGluValLeuAspAspLeu505560IleLysPheGluGluArgValGluLysMetAsnIleSerGluLeuIle65707580ArgGluIleLysAsnLysAspTyrAlaValArgLeuGluGlyThrAsp859095AspAsnSerIleThrLysLeuIleIleAspValAspAsnAspGlyAsn100105110GluTyrValIleSerGluSerLysAsnGluSerIleAlaGluLysPhe115120125AlaSerThrPheLysAsnGlyTrpAsnLysGluTyrGluAspGluGlu130135140GluPheTyrAsnAspMetGlnSerIleIleLeuLysSerGluLeuAsn14515015516015786PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽157MetAsnLeuLysGluLeuValArgGluIleLysAsnLysAspTyrThr151015AlaLysLeuSerGlyThrAspSerAsnSerIleThrGlnLeuIleIle202530HisValAsnAsnAspGlyAsnGluTyrGlyIleSerGluSerAsnPhe354045GluSerIleValGluLysPheValSerThrPheGluAsnGlyTrpAsp505560GlyAlaTyrGluAspGluGluGluPheTyrAsnAspMetGlnAspIle65707580ValAsnArgHisPheLys8515886PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽158MetAsnLeuLysGluLeuValArgGluIleLysAsnLysAspTyrThr151015AlaLysLeuSerGlyThrAspSerAsnSerIleThrGlnLeuIleIle202530HisValAsnAsnAspGlyAsnGluTyrGlyIleSerGluSerAsnPhe354045GluSerIleValGluLysPheValSerAsnPheGluAsnGlyTrpAsp505560GlyAlaTyrGluAspGluGluGluPheTyrAsnAspMetGlnAspIle65707580ValAsnArgHisPheLys85159151PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽159MetLysIleAsnGluLeuValArgGluIleLysSerArgAspTyrThr151015ValArgLeuAsnGlyThrAspSerAsnSerIleThrLysLeuIleIle202530AspValAsnAsnAspGlyAsnGluTyrValIleSerGluArgGlnAsp354045ThrSerIleValGluSerPheAlaAspSerPheIleAspGlyTrpThr505560GlyThrTyrGluAspGluGluAspPheTyrAsnAspMetGlnGluIle65707580AlaGlnAspIleIleLeuGluThrLeuLysGluAlaPheGluAsnAsn859095AsnTyrAsnThrAspGluValAspThrAspLeuPheAspGlyTyrGln100105110IleLysLeuAlaMetGluTyrAspAsnIleGlyGluLeuAlaThrSer115120125ValAsnLysThrLysHisPheThrAlaTyrMetAspAlaSerThrAsp130135140PheMetIleIleGluLysTyr145150160147PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽160MetSerIleIleAlaIleLysLysGluIleHisAlaLysGlyTyrLys151015ValThrGlyThrHisGlnGlyTyrIleAlaGlnIleAsnPheAspGly202530ThrGlyAsnGluTyrProLeuProAlaThrTrpAspGluPheIleGlu354045ThrPheLysAspGlyTrpAsnGlyThrTyrGluAspGluGlnAlaPhe505560PheAsnAspMetGlnGluValAlaLeuLysGluIleLeuAspGluLeu65707580ThrGlyAlaLeuPheCysGlnAspIleThrThrTyrAspPheThrIle859095AspAspValLysLysLysValIleThrLeuAspLysProThrPheGlu100105110GluAspAlaGluAspLeuIleIleGluPheAspSerThrCysPheTrp115120125AspAlaThrValGluAsnAspLysIleLysIleThrValArgAsnLys130135140SerArgTyr14516199PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽161MetSerIleIleAlaIleLysLysGluIleHisAlaLysGlyTyrLys151015ValThrGlyThrHisGlnGlyTyrIleAlaGlnIleAsnPheAspGly202530ThrGlyAsnGluTyrProLeuProAlaThrTrpAspGluPheIleGlu354045ThrPheLysAspGlyTrpAsnGlyThrTyrGluAspGluGlnAlaPhe505560PheAsnAspMetGlnGluIleAlaLeuAspGluIleLeuAspGluLeu65707580IleAspValLeuTyrAsnLeuAspIleThrThrTyrAsnPheThrIle859095AspAspSer162147PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽162MetSerIleIleAlaIleLysLysGluIleHisAlaLysGlyTyrLys151015ValThrGlyThrHisGlnGlyTyrIleAlaGlnIleAsnPheAspGly202530ThrGlyAsnGluTyrProLeuProAlaThrTrpAspGluPheIleGlu354045ThrPheLysAspGlyTrpAsnGlyThrTyrGluAspGluGlnAlaPhe505560PheAsnAspMetGlnGluIleAlaLeuGluGluIleLeuAspGluLeu65707580ThrGlyAlaLeuPheCysGlnAspIleThrThrTyrAspPheThrIle859095AspAspValLysLysLysValIleThrLeuAspLysProThrPheGlu100105110GluAspAlaGluAspLeuIleIleGluPheAspSerThrCysPheTrp115120125AspAlaThrValGluAsnAspLysIleLysIleThrValArgAsnLys130135140SerArgTyr145163149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽163MetSerIleIleAlaIleAsnLysGluIleArgAlaLysGlyTyrLys151015ValThrGlyThrHisGlnGlyTyrIleAlaGlnIleAsnPheGluGly202530ThrGlyAsnGluTyrProLeuProAlaThrTrpAspGluPheIleGlu354045ThrPheLysAspGlyTrpAsnGlyThrTyrGluAspGluGlnAlaPhe505560PheAsnAspMetGlnGluIleAlaLeuAspGluIleLeuAspGluLeu65707580IleAspValLeuTyrAsnLeuAspIleThrThrTyrAsnPheThrIle859095AspAspValLysLysLysValIleThrLeuAsnLysProIleAspGlu100105110GluGluThrGluAspLeuValGlnGluPheAsnValThrCysPheTrp115120125AspAlaThrValGluAspAspLysValLysValThrIleArgAsnLys130135140AsnArgAlaIleSer145164146PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽164MetSerThrThrAlaIleAsnLysGluIleHisAlaLysGlyTyrLys151015ValThrGlyThrHisGlnGlyTyrIleAlaGlnIleAsnPheAspGly202530ThrGlyAsnGluTyrProLeuProAlaThrTrpGluLysPheIleGlu354045ThrPheLysAspGlyTrpAspGlyThrTyrGluAspGluGlnAlaPhe505560PheAsnAspMetGlnGluIleAlaLeuAspGluIleLeuAspGluLeu65707580IleAspValLeuTyrAsnLeuAspIleThrThrTyrAsnPheThrIle859095AspAspValLysLysLysValIleThrLeuAsnLysProIleAspGlu100105110GluGluThrGluAspLeuValGlnGluPheAsnValThrCysPheTrp115120125AsnAlaIleValGluAspAspLysValLysIleThrValArgAsnLys130135140SerLys145165146PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽165MetSerThrThrAlaIleAsnLysGluIleHisAlaLysGlyTyrLys151015ValThrGlyThrHisGlnGlyTyrIleAlaGlnIleAsnPheAspGly202530ThrGlyAsnGluTyrProLeuProAlaThrTrpGluGluPheIleGlu354045ThrPheLysAspGlyTrpAspGlyThrTyrGluAspGluGlnAlaPhe505560PheAsnAspMetGlnGluIleAlaLeuAspGluIleLeuAspGluLeu65707580IleAspValLeuTyrAsnLeuAspIleThrThrTyrAsnPheThrIle859095AspAspValLysLysLysValIleThrLeuAsnLysProIleAspGlu100105110GluGluThrGluAspLeuValGlnGluPheAsnValThrCysPheTrp115120125AsnAlaIleValGluAspAspLysValLysIleThrValArgAsnLys130135140SerLys145166146PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽166MetSerThrThrAlaIleAsnLysGluIleHisAlaLysGlyTyrLys151015ValThrGlyThrHisGlnGlyTyrValAlaGlnIleAsnPheAspGly202530ThrGlyAsnGluTyrProLeuProAlaThrTrpGluGluPheIleGlu354045ThrPheLysAspGlyTrpAspGlyThrTyrLysAspGluGlnAlaPhe505560PheAsnAspMetGlnGluIleAlaLeuAspGluIleLeuAspGluLeu65707580IleAspThrLeuTyrAsnLeuAspIleThrThrTyrAspPheThrIle859095AspAspIleLysLysLysValIleThrLeuAspLysProThrAspArg100105110GluGluThrGluAspLeuValGlnGluPheAsnValThrCysPheTrp115120125AsnAlaIleValGluAspAspLysValLysValThrValArgAsnLys130135140SerLys145167130PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽MISC_FEATURE112..112Xaa是任何氨基酸167MetThrGlyThrHisGlnGlyTyrIleAlaGlnIleAsnPheAspGly151015ThrGlyAsnGluTyrProLeuProAlaThrTrpAspGluPheIleGlu202530ThrPheLysAspGlyTrpAsnGlyThrTyrGluAspGluGlnAlaPhe354045PheAsnAspMetGlnGluValAlaLeuLysGluIleLeuAspGluLeu505560IleAspValLeuTyrAsnLeuAspIleThrThrTyrAsnPheThrIle65707580AspAspValLysLysLysValIleThrLeuAsnLysProThrAspGlu859095GluAspAlaGluAspLeuValIleGluPheAspSerThrCysPheXaa100105110AspAlaThrValGluAsnAspLysIleLysValThrValArgAsnLys115120125SerLys130168149PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽168MetSerThrThrAlaIleAsnLysGluIleHisAlaLysGlyTyrLys151015ValThrGlyThrHisGlnGlyTyrMetAlaGlnIleAsnPheAspGly202530ThrGlyAsnGluPheProLeuProAlaThrTrpGluGluPheIleGlu354045ThrPheLysAspGlyTrpAspGlyThrTyrGluAspGluGlnAlaPhe505560PheAsnAspMetGlnGluValAlaLeuGluGluLeuLeuAspGluLeu65707580ThrAspValPheTyrAsnLeuAspIleThrAlaTyrAspPheThrVal859095AspAspValLysLysLysValIleThrLeuAspLysProThrAspArg100105110GluGluThrGluAspLeuValGlnGluPheLysAlaThrCysPheTrp115120125AsnAlaValValGluAspAspLysValLysValThrIleArgAsnLys130135140AsnArgAlaIleSer145169119PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽169MetGlnPheValValThrAsnLysSerGluLeuPheLysPheAlaTrp151015LysIlePheLysAlaAsnLysAspIleAlaPheSerGluCysLeuGln202530AsnAlaTrpPheGlnTyrLysArgTyrLeuAsnArgGluAlaIleLys354045AlaAlaGlnGlnArgLysLeuAlaLysPheIleAlaAspThrGluAsn505560GluGluValLysAlaTrpAsnTrpAlaGluLysLysLeuGlyValAla65707580LeuAsnLeuThrAspAlaGluLysGluArgAsnValArgAsnMetTyr859095LysGluMetTrpAsnAlaAsnValTrpAlaThrAlaIleLysAlaVal100105110LysLeuHisMetGluIleGly115170148PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽170MetAsnGluLeuArgSerLeuGluMetSerIleAsnAlaLysLysTyr151015AspThrArgLeuGluSerGlyAsnArgValLeuAsnIleGlyPheGly202530AspGlyGluAspTyrProValCysSerSerSerArgTyrSerLeuLys354045GluSerPheIleGluCysPheLysAspGlyTrpSerGlyThrTyrArg505560AspGluLysGluLeuMetGluAspMetGlnGluIleAlaGlnGluLeu65707580IleLeuGluGluLeuThrAspIlePheGluTyrTyrGluPheAsnThr859095AspGluIleAspThrAspLeuPheLysGlyPheThrPheAspValAsp100105110SerAspLeuGluAspSerMetAlaLeuMetLysAlaIleAsnAlaThr115120125LysTyrPheGluAlaArgSerSerSerTrpTyrAlaSerPheGluVal130135140SerTyrIleGly14517128RNA人工序列合成的crRNA间隔子序列171gauagcaagauggauaguuaguuuuaga2817236DNA人工序列合成的前间区序列phiJ0161a原噬菌体172acgctccgtaactatccatcttgctatctgtatatg3617336DNA人工序列合成的前间区序列phiJ0161a原噬菌体-互补序列173catatacagatagcaagatggatagttacggagcgt3617430DNA人工序列合成的间隔子序列，来自图7B174tggttctttcgctttcacaagatacttttg3017530DNA人工序列合成的间隔子序列，来自图7B175acccgtttctgatgaacgatacactgttgt3017630DNA人工序列合成的间隔子序列，来自图7B176catctatcaagctacaaattggatgtacac3017730DNA人工序列合成的间隔子序列，来自图7B177aagacgaagtgaacgaagagtctttcccag3017830DNA人工序列合成的间隔子序列，来自图7B178taacgtttggaaccctaggcattatgttgc3017930DNA人工序列合成的间隔子序列，来自图7B179gagaatgcaagaattaattaatgagtacag3018030DNA人工序列合成的间隔子序列，来自图7B180tgttgttataaacactgttaactgttgttc3018130DNA人工序列合成的间隔子序列，来自图7B181tctatgccttgatattttaaatgaccgagt3018230DNA人工序列合成的间隔子序列，来自图7B182taagcaagttgcagataagaaaaccatgtc3018330DNA人工序列合成的间隔子序列，来自图7B183ttttgatgatgaaccagaatggatttatta3018430DNA人工序列合成的间隔子序列，来自图7B184ctatccgcaaacacatgttgatggcgtcgt3018530DNA人工序列合成的间隔子序列，来自图7B185aaaaaaatattaacacgaattagatactaa3018630DNA人工序列合成的间隔子序列，来自图7B186caaggcacttttcattttttgaacacgcca3018730DNA人工序列合成的间隔子序列，来自图7B187aactgttgtgccttcctttgcgtgcacttg3018830DNA人工序列合成的间隔子序列，来自图7B188tctttttgatgtattcatttattgttagaa3018930DNA人工序列合成的间隔子序列，来自图7B;包括自靶向间隔子189agcatatacagatagcaagatggatagtta3019030DNA人工序列合成的间隔子序列，来自图7B190ttcaaggtcaagatcaagagcaagaatgcc3019130DNA人工序列合成的间隔子序列，来自图7B191cgtcgcataatactgttcctctgccgttcg3019229DNA人工序列合成的间隔子序列，来自图7B192gaatattcttccccccatgactaacaggg2919336DNA人工序列合成的间隔子序列，来自图7B193gttttagagctatgttattttgaatgctaccaaaac3619438RNA人工序列合成的crRNA间隔子序列194agcauauacagauagcaagauggauaguuaguuuuaga3819546DNA人工序列合成的靶DNA序列195acgctccgtaactatccatcttgctatctgtatatgcttttgcaga4619646DNA人工序列合成的靶DNA序列互补序列）196tctgcaaaagcatatacagatagcaagatggatagttacggagcgt46197154DNA酿脓链球菌Streptocococcuspyogenes197tatggctgataaatttctttgaatttctccttgattatttgttataaaagttataaaata60atcttgttggaaccattcaaaacagcatagcaagttaaaataaggctagtccgttatcaa120cttgaaaaagtggcaccgagtcggtgcttttttt154198157DNA单核细胞增生李斯特菌Listeriamonocytogenes198catattcttcatcccttcaacctgtttttagttgcattctttttataatttggtacaatg60tatatattgttagtattcaaaataacatagcaagttaaaataaggctttgtccgttatca120acttttaattaagtagcgctgtttcggcgcttttttt157199157DNA单核细胞增生李斯特菌Listeriamonocytogenes199catattcttcatcccttcaacctgtttttagttgcattctttttataatttggtacaatg60tatatattgttagtattcaaaataacatagcaagttaaaataaggctttgtccgttatca120acttttaattaagtagcgctgtttcggcgcttttttt157200157DNA无害李斯特菌Listeriainnocua200catattcttcatcccttcaacctatttttagttgcattctttttataatttggtacaatg60tatatattgttagtattcaaaataacatagcaagttaaaataaggctttgtccgttatca120acttttaattaagtagcgctgtttcggcgcttttttt157201246DNA酿脓链球菌Streptocococcuspyogenes201ttagatgaagattatttcttaataactaaaaatatggtataatactcttaataaatgcag60taatacaggggcttttcaagactgaagtctagctgagacaaatagtgcgattacgaaatt120ttttagacaaaaatagtctacgaggttttagagctatgctgttttgaatggtcccaaaac180tgcgctggttgatttcttcttgcgctttttgttttagagctatgctgttttgaatggtcc240caaaac246202233DNA单核细胞增生李斯特菌Listeriamonocytogenes202taaggtaaccgctgttctttgaaaacaaaataaattttatgtaaaccataaaatagcatt60caaaattgaaatcttgctatggatgaatggcgcgattacggaatcttggaggaaagaaaa120aattctgcgaggttttagagctatgttattttgaatgctaccaaaactcgtgatttcttc180ctcatgcgcgcttttgagttttagagctatgttattttgaatgctaccaaaac233203233DNA单核细胞增生李斯特菌Listeriamonocytogenes203taaggtaaccgctgttctttgaaaacaaaataaattttatgtaaaccataaaatagcatt60caaaattgaaatcttgctatggatgaatggcgcgattacggaatcttggaggaaagaaaa120aattctgcgaggttttagagctatgttattttgaatgctaccaaaactggttctttcgct180ttcacaagatacttttggttttagagctatgttattttgaatgctaccaaaac233204231DNA无害李斯特菌Listeriainnocua204tagagttcaaatgatctttgaaaactaaatagattttatgtgaaccataaaataagcatt60caaaattgaaatcttgctatggatgaatggcgcgattacgaaatctaggagaataaaaaa120ttctacgaggttttagagctatgttattttgaatgctaacaaaaccgtagcgctttggta180actttgcctaggatagttttagagctatgttattttgaatgctaacaaaac2312051368PRT酿脓链球菌Streptocococcuspyogenes205MetAspLysLysTyrSerIleGlyLeuAspIleGlyThrAsnSerVal151015GlyTrpAlaValIleThrAspGluTyrLysValProSerLysLysPhe202530LysValLeuGlyAsnThrAspArgHisSerIleLysLysAsnLeuIle354045GlyAlaLeuLeuPheAspSerGlyGluThrAlaGluAlaThrArgLeu505560LysArgThrAlaArgArgArgTyrThrArgArgLysAsnArgIleCys65707580TyrLeuGlnGluIlePheSerAsnGluMetAlaLysValAspAspSer859095PhePheHisArgLeuGluGluSerPheLeuValGluGluAspLysLys100105110HisGluArgHisProIlePheGlyAsnIleValAspGluValAlaTyr115120125HisGluLysTyrProThrIleTyrHisLeuArgLysLysLeuValAsp130135140SerThrAspLysAlaAspLeuArgLeuIleTyrLeuAlaLeuAlaHis145150155160MetIleLysPheArgGlyHisPheLeuIleGluGlyAspLeuAsnPro165170175AspAsnSerAspValAspLysLeuPheIleGlnLeuValGlnThrTyr180185190AsnGlnLeuPheGluGluAsnProIleAsnAlaSerGlyValAspAla195200205LysAlaIleLeuSerAlaArgLeuSerLysSerArgArgLeuGluAsn210215220LeuIleAlaGlnLeuProGlyGluLysLysAsnGlyLeuPheGlyAsn225230235240LeuIleAlaLeuSerLeuGlyLeuThrProAsnPheLysSerAsnPhe245250255AspLeuAlaGluAspAlaLysLeuGlnLeuSerLysAspThrTyrAsp260265270AspAspLeuAspAsnLeuLeuAlaGlnIleGlyAspGlnTyrAlaAsp275280285LeuPheLeuAlaAlaLysAsnLeuSerAspAlaIleLeuLeuSerAsp290295300IleLeuArgValAsnThrGluIleThrLysAlaProLeuSerAlaSer305310315320MetIleLysArgTyrAspGluHisHisGlnAspLeuThrLeuLeuLys325330335AlaLeuValArgGlnGlnLeuProGluLysTyrLysGluIlePhePhe340345350AspGlnSerLysAsnGlyTyrAlaGlyTyrIleAspGlyGlyAlaSer355360365GlnGluGluPheTyrLysPheIleLysProIleLeuGluLysMetAsp370375380GlyThrGluGluLeuLeuValLysLeuAsnArgGluAspLeuLeuArg385390395400LysGlnArgThrPheAspAsnGlySerIleProHisGlnIleHisLeu405410415GlyGluLeuHisAlaIleLeuArgArgGlnGluAspPheTyrProPhe420425430LeuLysAspAsnArgGluLysIleGluLysIleLeuThrPheArgIle435440445ProTyrTyrValGlyProLeuAlaArgGlyAsnSerArgPheAlaTrp450455460MetThrArgLysSerGluGluThrIleThrProTrpAsnPheGluGlu465470475480ValValAspLysGlyAlaSerAlaGlnSerPheIleGluArgMetThr485490495AsnPheAspLysAsnLeuProAsnGluLysValLeuProLysHisSer500505510LeuLeuTyrGluTyrPheThrValTyrAsnGluLeuThrLysValLys515520525TyrValThrGluGlyMetArgLysProAlaPheLeuSerGlyGluGln530535540LysLysAlaIleValAspLeuLeuPheLysThrAsnArgLysValThr545550555560ValLysGlnLeuLysGluAspTyrPheLysLysIleGluCysPheAsp565570575SerValGluIleSerGlyValGluAspArgPheAsnAlaSerLeuGly580585590ThrTyrHisAspLeuLeuLysIleIleLysAspLysAspPheLeuAsp595600605AsnGluGluAsnGluAspIleLeuGluAspIleValLeuThrLeuThr610615620LeuPheGluAspArgGluMetIleGluGluArgLeuLysThrTyrAla625630635640HisLeuPheAspAspLysValMetLysGlnLeuLysArgArgArgTyr645650655ThrGlyTrpGlyArgLeuSerArgLysLeuIleAsnGlyIleArgAsp660665670LysGlnSerGlyLysThrIleLeuAspPheLeuLysSerAspGlyPhe675680685AlaAsnArgAsnPheMetGlnLeuIleHisAspAspSerLeuThrPhe690695700LysGluAspIleGlnLysAlaGlnValSerGlyGlnGlyAspSerLeu705710715720HisGluHisIleAlaAsnLeuAlaGlySerProAlaIleLysLysGly725730735IleLeuGlnThrValLysValValAspGluLeuValLysValMetGly740745750ArgHisLysProGluAsnIleValIleGluMetAlaArgGluAsnGln755760765ThrThrGlnLysGlyGlnLysAsnSerArgGluArgMetLysArgIle770775780GluGluGlyIleLysGluLeuGlySerGlnIleLeuLysGluHisPro785790795800ValGluAsnThrGlnLeuGlnAsnGluLysLeuTyrLeuTyrTyrLeu805810815GlnAsnGlyArgAspMetTyrValAspGlnGluLeuAspIleAsnArg820825830LeuSerAspTyrAspValAspHisIleValProGlnSerPheLeuLys835840845AspAspSerIleAspAsnLysValLeuThrArgSerAspLysAsnArg850855860GlyLysSerAspAsnValProSerGluGluValValLysLysMetLys865870875880AsnTyrTrpArgGlnLeuLeuAsnAlaLysLeuIleThrGlnArgLys885890895PheAspAsnLeuThrLysAlaGluArgGlyGlyLeuSerGluLeuAsp900905910LysAlaGlyPheIleLysArgGlnLeuValGluThrArgGlnIleThr915920925LysHisValAlaGlnIleLeuAspSerArgMetAsnThrLysTyrAsp930935940GluAsnAspLysLeuIleArgGluValLysValIleThrLeuLysSer945950955960LysLeuValSerAspPheArgLysAspPheGlnPheTyrLysValArg965970975GluIleAsnAsnTyrHisHisAlaHisAspAlaTyrLeuAsnAlaVal980985990ValGlyThrAlaLeuIleLysLysTyrProLysLeuGluSerGluPhe99510001005ValTyrGlyAspTyrLysValTyrAspValArgLysMetIleAla101010151020LysSerGluGlnGluIleGlyLysAlaThrAlaLysTyrPhePhe102510301035TyrSerAsnIleMetAsnPhePheLysThrGluIleThrLeuAla104010451050AsnGlyGluIleArgLysArgProLeuIleGluThrAsnGlyGlu105510601065ThrGlyGluIleValTrpAspLysGlyArgAspPheAlaThrVal107010751080ArgLysValLeuSerMetProGlnValAsnIleValLysLysThr108510901095GluValGlnThrGlyGlyPheSerLysGluSerIleLeuProLys110011051110ArgAsnSerAspLysLeuIleAlaArgLysLysAspTrpAspPro111511201125LysLysTyrGlyGlyPheAspSerProThrValAlaTyrSerVal113011351140LeuValValAlaLysValGluLysGlyLysSerLysLysLeuLys114511501155SerValLysGluLeuLeuGlyIleThrIleMetGluArgSerSer116011651170PheGluLysAsnProIleAspPheLeuGluAlaLysGlyTyrLys117511801185GluValLysLysAspLeuIleIleLysLeuProLysTyrSerLeu119011951200PheGluLeuGluAsnGlyArgLysArgMetLeuAlaSerAlaGly120512101215GluLeuGlnLysGlyAsnGluLeuAlaLeuProSerLysTyrVal122012251230AsnPheLeuTyrLeuAlaSerHisTyrGluLysLeuLysGlySer123512401245ProGluAspAsnGluGlnLysGlnLeuPheValGluGlnHisLys125012551260HisTyrLeuAspGluIleIleGluGlnIleSerGluPheSerLys126512701275ArgValIleLeuAlaAspAlaAsnLeuAspLysValLeuSerAla128012851290TyrAsnLysHisArgAspLysProIleArgGluGlnAlaGluAsn129513001305IleIleHisLeuPheThrLeuThrAsnLeuGlyAlaProAlaAla131013151320PheLysTyrPheAspThrThrIleAspArgLysArgTyrThrSer132513301335ThrLysGluValLeuAspAlaThrLeuIleHisGlnSerIleThr134013451350GlyLeuTyrGluThrArgIleAspLeuSerGlnLeuGlyGlyAsp1355136013652061334PRT单核细胞增生李斯特菌Listeriamonocytogenes206MetLysAsnProTyrThrIleGlyLeuAspIleGlyThrAsnSerVal151015GlyTrpAlaValLeuThrAsnGlnTyrAspLeuValLysArgLysMet202530LysValAlaGlyAsnSerAspLysLysGlnIleLysLysAsnPheTrp354045GlyValArgLeuPheAspAspGlyGlnThrAlaValAspArgArgMet505560AsnArgThrAlaArgArgArgIleGluArgArgArgAsnArgIleSer65707580TyrLeuGlnGluIlePheAlaValGluMetAlaAsnIleAspAlaAsn859095PhePheCysArgLeuAsnAspSerPheTyrValAspSerGluLysArg100105110AsnSerArgHisProPhePheAlaThrIleGluGluGluValAlaTyr115120125HisLysAsnTyrArgThrIleTyrHisLeuArgGluGluLeuValAsn130135140SerSerGluLysAlaAspLeuArgLeuValTyrLeuAlaLeuAlaHis145150155160IleIleLysTyrArgGlyAsnPheLeuIleGluGlyAlaLeuAspThr165170175LysAsnThrSerValAspGlyValTyrGluGlnPheIleGlnThrTyr180185190AsnGlnValPheMetSerAsnIleGluGluGlyThrLeuAlaLysVal195200205GluGluAsnIleGluValAlaAsnIleLeuAlaGlyLysPheThrArg210215220ArgGluLysPheGluArgIleLeuGlnLeuTyrProGlyGluLysSer225230235240ThrGlyMetPheAlaGlnPheIleSerLeuIleValGlySerLysGly245250255AsnPheGlnLysValPheAspLeuIleGluLysThrAspIleGluCys260265270AlaLysAspSerTyrGluGluAspLeuGluAlaLeuLeuAlaIleIle275280285GlyAspGluTyrAlaGluLeuPheValAlaAlaLysAsnThrTyrAsn290295300AlaValValLeuSerSerIleIleThrValThrAlaThrGluThrAsn305310315320AlaLysLeuSerAlaSerMetIleGluArgPheAspAlaHisGluLys325330335AspLeuGlyGluLeuLysAlaPheIleLysLeuHisLeuProLysGln340345350TyrGlnGluIlePheAsnAsnAlaAlaIleAspGlyTyrAlaGlyTyr355360365IleAspGlyLysThrLysGlnValAspPheTyrLysTyrLeuLysThr370375380IleLeuGluAsnIleGluGlyAlaAspTyrPheIleAlaLysIleGlu385390395400GluGluAsnPheLeuArgLysGlnArgThrPheAspAsnGlyAlaIle405410415ProHisGlnLeuHisLeuGluGluLeuGluAlaIleIleHisGlnGln420425430AlaLysTyrTyrProPheLeuArgGluAspTyrGluLysIleLysSer435440445LeuValThrPheArgIleProTyrPheValGlyProLeuAlaLysGly450455460GlnSerGluPheAlaTrpLeuThrArgLysAlaAs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GlnGlyLysThrSerTyr530535540PheSerGlyGlnGluLysGluGlnIlePheAsnAspLeuPheLysGln545550555560LysArgLysValLysLysLysAspLeuGluLeuPheLeuArgAsnMet565570575SerHisValGluSerProThrIleGluGlyLeuGluAspSerPheAsn580585590SerSerTyrSerThrTyrHisAspLeuLeuLysValGlyIleLysGln595600605GluIleLeuAspAsnProValAsnThrGluMetLeuGluAsnIleVal610615620LysIleLeuThrValPheGluAspLysArgMetIleLysGluGlnLeu625630635640GlnGlnPheSerAspValLeuAspGlyValValLeuLysLysLeuGlu645650655ArgArgHisTyrThrGlyTrpGlyArgLeuSerAlaLysLeuLeuMet660665670GlyIleArgAspLysGlnSerHisLeuThrIleLeuAspTyrLeuMet675680685AsnAspAspGlyLeuAsnArgAsnLeuMetGlnLeuIleAsnAspSer690695700AsnLeuSerPheLysSerIleIleGluLysGluGlnValThrThrAla705710715720AspLysAspIleGlnSerIleValAlaAspLeuAlaGlySerProAla725730735IleLysLysGlyIleLeuGlnSerLeuLysIleValAspGluLeuVal740745750SerValMetGlyTyrProProGlnThrIleValValGluMetAlaArg755760765GluAsnGlnThrThrGlyLysGlyLysAsnAsnSerArgProArgTyr770775780LysSerLeuGluLysAlaIleLysGluPheGlySerGlnIleLeuLys785790795800GluHisProThrAspAsnGlnGluLeuArgAsnAsnArgLeuTyrLeu805810815TyrTyrLeuGlnAsnGlyLysAspMetTyrThrGlyGlnAspLeuAsp820825830IleHisAsnLeuSerAsnTyrAspIleAspHisIleValProGlnSer835840845PheIleThrAspAsnSerIleAspAsnLeuValLeuThrSerSerAla850855860GlyAsnArgGluLysGlyAspAspValProProLeuGluIleValArg865870875880LysArgLysValPheTrpGluLysLeuTyrGlnGlyAsnLeuMetSer885890895LysArgLysPheAspTyrLeuThrLysAlaGluArgGlyGlyLeuThr900905910GluAlaAspLysAlaArgPheIleHisArgGlnLeuValGluThrArg915920925GlnIleThrLysAsnValAlaAsnIleLeuHisGlnArgPheAsnTyr930935940GluLysAspAspHisGlyAsnThrMetLysGlnValArgIleValThr945950955960LeuLysSerAlaLeuValSerGlnPheArgLysGlnPheGlnLeuTyr965970975LysValArgAspValAsnAspTyrHisHisAlaHisAspAlaTyrLeu980985990AsnGlyValValAlaAsnThrLeuLeuLysValTyrProGlnLeuGlu99510001005ProGluPheValTyrGlyAspTyrHisGlnPheAspTrpPheLys101010151020AlaAsnLysAlaThrAlaLysLysGlnPheTyrThrAsnIleMet102510301035LeuPhePheAlaGlnLysAspArgIleIleAspGluAsnGlyGlu104010451050IleLeuTrpAspLysLysTyrLeuAspThrValLysLysValMet105510601065SerTyrArgGlnMetAsnIleValLysLysThrGluIleGlnLys107010751080GlyGluPheSerLysAlaThrIleLysProLysGlyAsnSerSer108510901095LysLeuIleProArgLysThrAsnTrpAspProMetLysTyrGly110011051110GlyLeuAspSerProAsnMetAlaTyrAlaValValIleGluTyr111511201125AlaLysGlyLysAsnLysLeuValPheGluLysLysIleIleArg113011351140ValThrIleMetGluArgLysAlaPheGluLysAspGluLysAla114511501155PheLeuGluGluGlnGlyTyrArgGlnProLysValLeuAlaLys116011651170LeuProLysTyrThrLeuTyrGluCysGluGluGlyArgArgArg117511801185MetLeuAlaSerAlaAsnGluAlaGlnLysGlyAsnGlnGlnVal119011951200LeuProAsnHisLeuValThrLeuLeuHisHisAlaAlaAsnCys120512101215GluValSerAspGlyLysSerLeuAspTyrIleGluSerAsnArg122012251230GluMetPheAlaGluLeuLeuAlaHisValSerGluPheAlaLys123512401245ArgTyrThrLeuAlaGluAlaAsnLeuAsnLysIleAsnGlnLeu125012551260PheGluGlnAsnLysGluGlyAspIleLysAlaIleAlaGlnSer126512701275PheValAspLeuMetAlaPheAsnAlaMetGlyAlaProAlaSer128012851290PheLysPhePheGluThrThrIleGluArgLysArgTyrAsnAsn129513001305LeuLysGluLeuLeuAsnSerThrIleIleTyrGlnSerIleThr131013151320GlyLeuTyrGluSerArgLysArgLeuAspAsp13251330209132PRT未知未知细菌,未知病毒噬菌体或合成的Cas9抑制性多肽209MetGlyMetThrThrAlaArgLysLysPheTyrGlnAlaIleSerGlu151015PheGluAlaMetThrGlyLysAspValGluArgThrProGlnIleAla202530AspGluValLeuAsnAspAlaGluTyrIleAlaPheThrLysThrGlu354045LysTyrAlaLeuTyrLeuCysThrSerAsnValGluGlyLeuGluAsp505560ArgTyrPheLeuAspGluGluCysLeuAspSerThrPheLeuGluThr65707580GluAspAsnGluThrTyrTyrIleHisPheLeuGlnGluThrGluPhe859095SerGluAspAspAsnGluAspGluLeuProLeuAlaThrGluGluGln100105110IleGluAlaTyrAspLysGlnGluGluLeuLysAlaValIleLeuLys115120125LysGluLeuAsn130

权利要求：1.一种抑制细胞中Cas9多肽的方法，所述方法包括，将Cas9抑制性多肽引入细胞，其中：所述Cas9抑制性多肽对于所述细胞而言是异源性的，并且所述Cas9抑制性多肽与SEQIDNO:1-170中任一或多个基本例如，至少60％，70％，80％，90％，95％相同；由此抑制细胞中的Cas9多肽。2.如权利要求1.一种抑制细胞中Cas9多肽的方法，所述方法包括，所述的方法，包括，使所述Cas9抑制性多肽与细胞中的Cas9多肽接触。3.如权利要求1.一种抑制细胞中Cas9多肽的方法，所述方法包括，所述的方法，其中，所述Cas9抑制性多肽包含SEQIDNO:1-170之一。4.如权利要求1.一种抑制细胞中Cas9多肽的方法，所述方法包括，所述的方法，其中，在所述引入之前，所述细胞包含所述Cas9多肽。5.如权利要求4所述的方法，其中，所述细胞包含表达盒，该表达盒含有操作性连接至编码所述Cas9多肽的多核苷酸的启动子。6.如权利要求5所述的方法，其中，所述启动子是诱导型的，且所述方法包括，在所述引入之前，使所述细胞与诱导Cas9多肽在所述细胞中表达的试剂或条件接触。7.如权利要求1.一种抑制细胞中Cas9多肽的方法，所述方法包括，所述的方法，其中，在所述引入之后，所述细胞包含Cas9多肽。8.如权利要求7所述的方法，其中，所述启动子是诱导型的，且所述方法包括，在所述引入之后，使所述细胞与诱导Cas9多肽在所述细胞中表达的试剂或条件接触。9.如权利要求1.一种抑制细胞中Cas9多肽的方法，所述方法包括，所述的方法，其中，所述引入包括，使所述Cas9抑制性多肽在所述细胞中从表达盒表达，其存在于所述细胞中且对所述细胞而言是异源性的，其中，所述表达盒包含操作性连接至编码所述Cas9抑制性多肽的多核苷酸的启动子。10.如权利要求9所述的方法，其中，所述启动子是诱导型启动子，并且所述引入包括使所述细胞与诱导该Cas9抑制性多肽表达的试剂接触。11.如权利要求1.一种抑制细胞中Cas9多肽的方法，所述方法包括，所述的方法，其中，所述引入包括，将编码所述Cas9抑制性多肽的RNA引入所述细胞，并且，在细胞中从RNA表达该Cas9抑制性多肽。12.如权利要求1.一种抑制细胞中Cas9多肽的方法，所述方法包括，所述的方法，其中，所述引入包括，将所述Cas9抑制性多肽插入所述细胞或使所述细胞与所述Cas9抑制性多肽接触。13.如权利要求1.一种抑制细胞中Cas9多肽的方法，所述方法包括，-12中任一项所述的方法，其中，所述细胞是真核细胞。14.如权利要求13所述的方法，其中，所述细胞是哺乳动物细胞。15.如权利要求14所述的方法，其中，所述细胞是人细胞。16.如权利要求14-15中任一项所述的方法，其中，所述细胞是血液或诱导型多能干细胞。17.如权利要求14-16中任一项所述的方法，其中，所述方法离体进行。18.如权利要求17所述的方法，其中，在所述引入和接触之后，将所述细胞引入哺乳动物。19.如权利要求18所述的方法，其中，所述细胞对所述哺乳动物而言是自体同源的。20.如权利要求1.一种抑制细胞中Cas9多肽的方法，所述方法包括，-12中任一项所述的方法，其中，所述细胞是原核细胞。21.一种包含Cas9抑制性多肽的细胞，其中，所述Cas9抑制性多肽对于所述细胞而言是异源性的，并且所述Cas9抑制性多肽与SEQIDNO:1-170中任一或多个基本相同。22.如权利要求21所述的细胞，其中，所述细胞是真核细胞。23.如权利要求22所述的方法，其中，所述细胞是哺乳动物细胞。24.如权利要求23所述的方法，其中，所述细胞是人细胞。25.如权利要求21所述的方法，其中，所述细胞是原核细胞。26.一种包含编码Cas9抑制性多肽的核酸的多核苷酸，其中，所述Cas9抑制性多肽与SEQIDNO:1-170中任一或多个基本相同。27.如权利要求26所述的多核苷酸，其包含表达盒，所述表达盒包含操作性连接至所述核酸的启动子。28.如权利要求27所述的多核苷酸，其中，所述启动子对于编码该Cas9抑制性多肽的多核苷酸而言是异源性的。29.如权利要求27或28所述的多核苷酸，其中，所述启动子是诱导型的。30.如权利要求26所述的多核苷酸，其中，所述多核苷酸是DNA或RNA。31.一种载体，其包含如权利要求27-29中任一项所述的表达盒。32.如权利要求31所述的载体，其中，所述载体是病毒载体。33.一种分离的Cas9抑制性多肽，其中，所述Cas9抑制性多肽与SEQIDNO:1-170中任一或多个基本相同。34.一种药物组合物，其包含如权利要求26-29中任一项所述的多核苷酸或如权利要求33所述的多肽。35.一种递送载剂，其包含如权利要求26-30中任一项所述的多核苷酸或如权利要求33所述的多肽。36.如权利要求35所述的递送载剂，其中，所述递送载剂是脂质体或纳米颗粒。

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